gene editing

CRISPR-Based Therapeutic Approaches in Lung Cancer

This research paper explores the potential of CRISPR-based therapeutic approaches in the diagnosis, treatment, and management of lung cancer. It examines how CRISPR-Cas genome-editing technologies can be used to identify and modify cancer-associated genes, investigate molecular mechanisms of tumor development, and deve...

Regulatory divergence of duplicated VIH genes links phosphate signaling to nodule development in Medicago truncatula.

Primary metabolites and their derivatives often serve as intracellular signals. Inositol pyrophosphates are central regulators of phosphate signaling, but their roles in legume nodulation remain poorly understood. Here, we identify two conserved Vip1 Homolog/Diphosphoinositol Pentakisphosphate Kinase genes, MtVIH1 and...

Nanocarrier-Based Approaches for Intracellular Protein Transport in Therapeutic Applications

Intracellular protein delivery is becoming a breakthrough tool in biotechnology and medicine, opening new possibilities for gene editing, enzyme replacement, immunomodulation, and targeted therapies. Traditional techniques like microinjection and electroporation can deliver proteins into the cytosol, but their low thro...

Cas12a cleavage and trimming kinetics reveal mismatches as a strategy to steer editing

This work investigated Cas12a’s ability to produce different ends and if they can be used to redistribute editing outcomes, and exploited the differences in cleavage kinetics between matched and mismatched gRNAs to develop reprogrammed gRNAs, i.e. rpgRNAs.

Microbes and Medicine: Revolutionizing Healthcare with Biotech and Pharma

This chapter outlines the significant contributions that pharmaceutical biotechnology and microbiology have made to medication development and healthcare. Using biological systems and living beings, biotechnology creates novel goods, particularly in the medical field. On the other hand, by examining microorganisms esse...

Advances in Organoids for Drug Development: From Discovery to Delivery

Organoid technology has emerged as a promising platform for drug development by enabling the in vitro reconstruction of human tissue architecture, cellular heterogeneity, and key physiological functions. Compared with conventional two-dimensional cell cultures and animal models, organoids provide improved physiological...

Establishing stable non-diapause egg production in p50ma and f38 strains for genome engineering by diapause hormone receptor knockout in Bombyx mori (Lepidoptera: Bombycidae)

Molecular Scissors With a Search Bar: A Contemporary Synthesis Review of CRISPR and the Editing of the Genome

This article presents a narrative review of CRISPR and the Editing of the Genome in the context of Biology. The literature on this topic has expanded substantially over recent decades, yet it remains fragmented across subfields, methods, and national research traditions. Drawing on an interpretive synthesis of represen...

Molecular Scissors With a Search Bar: A Critical Survey Review of CRISPR and the Editing of the Genome

This article presents a narrative review of CRISPR and the Editing of the Genome in the context of Biology. The literature on this topic has expanded substantially over recent decades, yet it remains fragmented across subfields, methods, and national research traditions. Drawing on an interpretive synthesis of represen...

Stem Cells As A Promising Therapeutic Strategy For Diabetes Mellitus: A Comprehensive Review

Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia resulting from impaired insulin secretion, insulin resistance, or both. Insulin and antidiabetic medications can control blood glucose levels well but cannot help recover dysfunctional pancreatic β-cells or change the pathological processe...

Rice leaf structural adjustment to sustain photosynthetic traits under drought and produce more grain: evidence from field studies of the 3K indica panel

Understanding plant traits that contribute to maintenance of physiological activities under drought is crucial for sustainable rice production. Leaf morphological characters together with UAV-based HTP measurements of canopy temperature (CT), NDVI, leaf water potential ( ψ Leaf ), photosynthetic traits like A, g s E an...

Groundnut Improvement for Aflatoxin Resistance: Progress and Opportunities

Groundnut, or peanut, is an industrial oilseed crop that serves the food and feed industries and provides income along the value chain. Pre‐ and post‐harvest aflatoxin contamination, caused by Aspergillus spp., hinders the food and feed value and market opportunities of groundnut products. Developing and deployin...