gene editing

Gene pyramiding in the era of genomics and genome editing: Advances, applications, and future perspectives for crop improvement

Design and validation of CRISPR/Cas9 sgRNAs and vector construction for targeting the LsBOS gene in grasspea (Lathyrus sativus L.)

Grasspea is an important grain legume crop due to its hardiness and high protein content. However, the presence of a neurotoxic compound β -ODAP, which is associated with lathyrism, limits its widespread cultivation and consumption. In this study, the LsBOS gene, which encodes β -ODAP synthase, was PCR-amplif...

Domesticated pennycress is a predominantly self-pollinated crop

Domesticated pennycress (Thlaspi arvense L.) is an emerging winter annual oilseed crop developed from field pennycress to deliver commercial agronomic performance, low-carbon feedstock for biofuel production, and value-added animal feed. Effective breeding, seed production, and stewardship of this newly domesticated cr...

ALA-TOO INTERNATIONAL UNIVERSITY The Therapeutic Potential and Biophysical Challenges of CRISPR-Cas9 Gene Editing in β-Thalassemia A Comprehensive Review of Mechanisms, Clinical Evidence and Translational Barriers

Evolutionary diversification and functional roles of MYB transcription factors in algae: insights from genomic distribution and regulatory networks-dataset

MYB transcription factors (TFs) represent a conserved family of DNA-binding regulators involved in diverse cellular and physiological processes. MYB TFs are well-characterized in terrestrial plants, but their evolutionary history and functional diversity in algae remain poorly understood. This review summarizes current...

Annotation files for the naked mole-rat (Heterocephalus glaber) telomere-to-telomere genome

Repo Summary: This repository contains the summary annotation files for the naked mole-rat (Heterocephalus glaber) telomere-to-telomere genome, mHetGla1.1, and the primary assembly for the Cape-dune mole-rat genome (Bathyergus suillus), mBatsui1.1-pri, resources. Materials and methods for all sequencing, assembly, anno...

Vitessce

Minor Changes Upgrade from Material UI (MUI) v7 to v9, and from MUI X Data Grid v8 to v9. Removed the unused @mui/lab dependency. (@vitessce/styles) (#2601) Patch Changes Add annotation story editing to the annotation controller (frames, text, view state capture, and shapes drawn on spatial/scatterplot views via a new...

The Missing Antigens of Neuroendocrine Tumors: A Null-Hypothesis Framework for Testing Microbial Control of Spatial Immune Editing

Well-differentiated gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are commonly regarded as immunologically “cold,” yet this label does not explain their marked spatial heterogeneity in immune infiltration, antigenpresentation machinery, stromal organization, and malignant-cell state. Intratumoral bacterial si...

FOXJ1, a novel oncosupressor transcription factor in a subgroup of PDAC patients

Pancreatic ductal adenocarcinoma (PDAC) is one of the most prevalent and deadliest cancers. It is predicted to become the second leading cause of cancer mortality within a decade, with an overall 5-year survival of 9%. The existing literature extensively supports the notion that epigenetic modifications and alterations...

Integrating Genomic Selection and CRISPR/Cas Editing for Predictive and Precision Wheat Breeding

Wheat (Triticum aestivum L.) is an important staple food and feed crop that makes a major contribution to global food and nutritional security, yet continued improvement is increasingly constrained by climate change, evolving pathogens, and the slow pace of conventional breeding.Recent evidence indicates that, in some...

Genomics and Bioinformatics for Next-Generation Crop Improvement: From Pangenomes to AI-Driven Precision Breeding

Crop genomics has been based on single reference genomes for over last two decades, which have been used for the development of markers, genome-wide association studies and genomic selection, but which have underrepresented structural variation, gene content and haplotype diversity within species.This review explores t...

An optimized pipeline for cell-based validation of gRNAs designs to inactivate coding and non-coding RNAs using CRISPR-Cas9

Genome-editing technologies based on bacterial Cas9 proteins have become a gold standard for introducing precise genetic modifications across multiple species and experimental models. These tools require the design and validation of guide RNAs (gRNAs) that direct the Cas9 protein to the actual genome location to be edi...