gene editing

Triple gene-knockout xenogeneic bone repair material achieves synergistic optimization of bone repair immunomicroenvironment and bone regeneration via antigen-immunity-inflammation axis modulation.

This study constructed a triple gene-edited and Urist-processed xenogeneic decalcified bone matrix system, offering an innovative gene-editing bone repair strategy to overcome immune rejection challenges in xenogeneic bone grafts and illuminates the therapeutic prospects of synergistically attenuating xenotransplantati...

CRISPR and Plant Metabolic Engineering: Opportunities for Enhancing Secondary Metabolite Production

The findings affirm that CRISPR is reshaping the landscape of plant biotechnology, offering sustainable routes for producing high-value metabolites for food, medicine and industry.

Toward decision-readiness for human germline gene editing:What if...

The possibility of altering DNA in human embryos (human germline gene editing), raises many questions. Changes in the DNA of germline cells would be passed on to next generations, thereby affecting humans as a species. CRISPR, the technology that is most promising for altering the DNA of embryos, has the potential to e...

Effective protoplast isolation and transient transformation system for verification of gene function and editing efficiency in radish

Cas12a chRDNA-mediated in vivo genome editing for high specificity functional gene disruption

CRISPR hybrid guides containing a combination of RNA and DNA nucleotides (CRISPR hybrid RNA-DNA or chRDNA) enhance both Cas9 and Cas12a nuclease specificity and reduce off-target editing in vitro. CRISPR-Cas9 with all-RNA crRNA guides has been implemented for in vivo intervention of familial hypercholesterolemia, an in...

Precision Genome Editing Strategies for Enduring Lipid Lowering in Atherosclerosis.

This review examines the significant transition in cardiovascular research from conventional, wide knockout models to the utilization of advanced precision genome editing methods, particularly emphasizing CRISPR-Cas9, base editing, and prime editing.

Inheritance of a Single Edited CD46 Allele Is Associated with Reduced Ex Vivo Susceptibility to Bovine Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) remains an economically important pathogen of cattle despite widespread vaccination. A homozygous CD46-edited Gir heifer (Ginger) was previously shown to have significantly reduced susceptibility to BVDV. The edited allele contains an in-frame six amino acid substitution within the vi...

Integrating multi-omics and genome editing for biotic stress resistance and quality improvement in groundnut

Groundnut ( Arachis hypogaea L.) is a major legume and oilseed crop, yet its productivity is constrained by biotic stresses, including fungal, bacterial, and viral pathogens and insect pests, which reduce yield and seed quality. These constraints are difficult to address through conventional breeding, because stress to...

Gene Therapy Approaches for Rare Genetic Diseases: Current Advances, Challenges and Future Perspectives

Rare genetic diseases are a highly heterogeneous group of disorders affecting a small number of patients but collectively pose a significant global health burden. Current treatments offer clinical benefits but often fail to correct molecular defects, reach tissues, avoid immunity, or eliminate repeated dosing. These li...

Unveiling the role of extracellular vesicles in HIV infection: molecular mechanisms, viral persistence, and therapeutic opportunities

The molecular mechanisms through which EVs have been proposed to contribute to HIV infection are examined, focusing on host–virus interactions, immune regulation, chronic inflammation, and viral latency.

Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing the Major Neutralizing Epitopes A and D of Transmissible Gastroenteritis Virus Spike Protein

A recombinant virus called rPRV-AD is built that carries key bits of the transmissible gastroenteritis virus (TGEV) spike protein that exhibited lower protective efficacy against TGEV than a commercial vaccine, while comparable protective observations were obtained against pseudorabies.

Specificity and Regulatory Mechanism of Metal Ions in Cas12a Enzymatic Cleavage Activity.

It was found that certain metal ions significantly affected both the cleavage activity and the reaction specificity of Cas12a, and this results provide a theoretical foundation and offer innovative perspectives for the application of Cas12a-based enzyme technology.