gene editing

A REVIEW ARTICLE: APPLICATIONS OF GENETIC ENGINEERING, RECOMBINANT DNA TECHNOLOGY, AND GENETIC IMPROVEMENT OF MEDICINAL AND AROMATIC PLANTS FOR FUTURE PROSPECTS

Medicinal and aromatic plants play a crucial role in benefiting humans and other animals directly and indirectly by providing the raw materials for pharmaceutical industries and curing severe diseases. These plants provide natural medicines and have great importance in sustaining the economy of several countries of the...

pgRNA-m6A levels associate with HBV replication and liver function

Site-specific m6A demethylation of pgRNA1907-m6A significantly reduced HBV DNA, hepatitis B e antigen (HBeAg) and HBV pgRNA, while induced levels of interferon alfa 2 (IFN-α2) and apolipoprotein B mRNA editing enzyme catalytic subunit 3A (APOBEC3A) were reduced.

Beyond immune rejection: integrative genetic engineering strategies for overcoming coagulopathy in xenotransplantation.

The four-decade evolution of genetically engineered (GE) pigs from the first α-1,3-galactosyltransferase knockout in 2003 to today's 10-gene and 69-edit donors has progressively dismantled the immunologic barriers to xenotransplantation. Hyperacute and acute humoral rejection have been largely overcome through triple k...

Editorial: Advances in phenomics, metabolomics, and genomics for precision breeding of ornamental horticultural crops

Peony represents one of the world's most valuable ornamental crops, prized for its aesthetic appeal and expanding commercial significance. However, conventional propagation methods remain slow and inefficient, limiting the rapid dissemination of elite cultivars and the implementation of precision breeding strategies. S...

Immune gene and protein profiles across blood, cerebrospinal fluid and peripheral nerve in inflammatory neuropathies

Public aggregate materials accompanying the Human Genomics submission manuscript v42. Version 2.3.0 includes the revised manuscript with five embedded main figures and three editable tables, updated figure labels and legends, the edited graphical abstract, four supplementary figures, a 49 worksheet aggregate data workb...

Enzymatic skeletal editing reaction forming the 1,3,5-triazine core during biosynthesis of the anticancer nucleoside analogue 5-azacytidine

Programmable RNA-Guided DNA Recombination: Mechanisms, Engineering, and Applications

The emergence of seekRNA- and bridgeRNA-guided recombination has introduced a distinct paradigm in genome engineering by coupling programmable RNA-directed DNA recognition with recombinase-mediated insertion, excision, inversion, and genomic rearrangement without canonical double-strand breaks. Since their discovery in...

Immune gene and protein profiles across blood, cerebrospinal fluid and peripheral nerve in inflammatory neuropathies

Public aggregate materials accompanying the Human Genomics submission manuscript v42. Version 2.3.0 includes the revised manuscript with five embedded main figures and three editable tables, updated figure labels and legends, the edited graphical abstract, four supplementary figures, a 49 worksheet aggregate data workb...

Genetically Modified and Gene-Edited Organisms—Objectives, Public Perception and Applications

Simple Summary The debate about the acceptance or rejection of genetically modified organisms (GMOs) has lasted for many decades. At the heart of this debate is the balance between the potential benefits of GMOs and concerns about their possible effects on human health, the environment, animal welfare and society. Over...

Transgenic overexpression of GmAPC7-CT improves seed yield and reduces susceptibility to soybean mosaic virus and Meloidogyne incognita in soybean

Stable transgenic soybean lines overexpressing the GmAPC7-CT gene have demonstrated increased seed yield and reduced susceptibility to the soybean mosaic virus and Meloidogyne incognita. The Anaphase-Promoting Complex subunit 7 (APC7) is a core structural component of the anaphase-promoting complex or cyclosome (APC/C)...

An improved protocol for high-efficiency and cost-effective CRISPR/Cas9-mediated knock-ins in C. elegans

This protocol provides a simplified CRISPR/Cas9-mediated knock-in pipeline suitable for editing a large number of loci, and offers cost-effective, alternative ssDNA donor preparation strategies, overcoming the requirement of expensive locus-specific, long phosphorylated primers.

pgRNA-m6A levels associate with HBV replication and liver function

m6A modification has been shown to play a role in regulating HBV RNA expression. However, it remains unclear whether the m6A levels of HBV pregenomic RNA (pgRNA) are related to HBV replication and liver function. Serum HBV pgRNA-m6A levels were determined by T3 DNA ligase assay. HBV DNA copy numbers and gene expression...