Construction and Immunogenicity of a Recombinant Pseudorabies Virus Expressing the Major Neutralizing Epitopes A and D of Transmissible Gastroenteritis Virus Spike Protein
TL;DR
A recombinant virus called rPRV-AD is built that carries key bits of the transmissible gastroenteritis virus (TGEV) spike protein that exhibited lower protective efficacy against TGEV than a commercial vaccine, while comparable protective observations were obtained against pseudorabies.
Abstract
Simple Summary Transmissible gastroenteritis and pseudorabies are two viral diseases that severely affect pig farms, causing substantial economic losses. Conventional vaccines have shown declining protective efficacy. We built a recombinant virus called rPRV-AD that carries key bits of the transmissible gastroenteritis virus (TGEV) spike protein. In the lab, it grew just like the original parent virus. When administered to 2-week-old piglets in this preliminary animal trial, the vaccinated animals remained clinically healthy and produced antibodies against both viruses. After virus challenge, vaccinated pigs exhibited milder clinical signs and reduced viral loads in intestines and feces. Under our experimental conditions, rPRV-AD exhibited lower protective efficacy against TGEV than a commercial vaccine, while comparable protective observations were obtained against pseudorabies.
