gene editing

Defending synthetic DNA orders against intra-order splitting-based obfuscation

Biosecurity screening of synthetic DNA orders is a key defense against malicious actors and careless enthusiasts producing dangerous pathogens or toxins. It is important to evaluate biosecurity screening tools for potential vulnerabilities and to work responsibly with providers to ensure that vulnerabilities can be pat...

Advances in plant metabolic engineering through synthetic biology

This review highlights the primary technologies used in plant metabolic engineering and focuses on representative examples of their implementation for crop improvement, including but not limited to CRISPR-Cas systems, multiplex genome editing, knock-in and knock-out strategies, CRISPR-mediated gene regulation, base edi...

Phytomedicinal approaches to postprandial hyperglycemia: Plants with α‑amylase and α‑glucosidase inhibitory effects (Review)

Diabetes mellitus (DM), a non-communicable disease with a high disease burden, is characterized by insulin resistance and postprandial hyperglycemia.An estimated 589 million adults worldwide were suffering from DM in 2024, a number projected to exceed 853 million by the year 2050.Diabetes has an evolving epidemiologica...

Wheat genetic resources for sodicity tolerance: from salinity-derived evidence toward sodicity-specific breeding

Disease Modeling of Fabry Disease Cardiomyopathy using Base Editing-Established Isogenic iPSCs and Application of Prime Editing for Gene Correction

Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation

Neu5Gc and beyond: sialylation at the innate immune and thromboinflammatory interface of xenotransplantation

Recent advances in genetic engineering have substantially reduced the major carbohydrate barriers to pig-to-human xenotransplantation. Deletion of GGTA1, CMAH, and B4GALNT2 decreases α Gal, Neu5Gc, and B4GALNT2-dependent Sda-like xenoantigens and thereby attenuates preformed antibody binding and complement-mediated...

A photostable version of HY5 confers tolerance to proximity shade and improved defense responses in tomato

The results indicate that the editing approach allows the generation of gain-of-function tomato plants in which HY5 is camouflaged to avoid COP1 recognition and eventual degradation, and provides a biotechnological tool to create more compact and pathogen-resistant plants amenable to high planting densities.

Artificially Engineered Carcinogenesis as a Biosecurity Threat: A Policy Review and Call for Inclusion in National and International Governance Frameworks

This paper calls for the explicit recognition of artificially engineered carcinogenesis as a potential biosecurity threat and proposes targeted policy measures to strengthen governance, including risk assessment frameworks, enhanced ethical review processes, and integration of oncogenic risk considerations into biosecu...

Stoichiometric m6A regulation of YY1 orchestrates the metabolic switch during cardiac development

Chemical modifications of RNA provide an additional regulatory layer essential for development. N6A methyladenosine (m6A) is the most abundant internal mRNA mark, but how site specificity, stoichiometry, and cellular context together determine mRNA fate remains unclear. Here, we define these rules in cardiogenesis by c...

The conserved function of bHLH121 and overexpression of N-terminal bHLH121 fragment suppresses iron deficiency response in Arabidopsis

Iron (Fe) homeostasis is fundamental to plant growth and development and is centrally regulated by a well-conserved network of basic helix-loop-helix (bHLH) transcription factors. In Arabidopsis thaliana, bHLH121 regulates the expression of FER-like iron deficiency-induced transcription factor (FIT) and clade Ib bHLH38...

Cargo size matters when designing lipid nanoparticles for delivery of large gene editors