gene editing

Endoplasmic reticulum-associated NcDYRK2 regulates Ca2⁺ dynamics, motility, and development gene expression in Neospora caninum

Neospora caninum is an obligate intracellular apicomplexan parasite and a leading cause of bovine abortion worldwide, imposing substantial economic losses on the cattle industry. Dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs) are highly conserved serine/threonine kinases that regulate diverse cellu...

Probing the Conformational Dynamics of VeCas9 Using High-Resolution Single-Molecule Junctions

By integrating VeCas9 into a graphene-molecule-graphene single-molecule junction platform, mechanistic insights into the Cas9 function are provided and the strong potential of high temporal-resolution single-molecule techniques for studying dynamic biological processes is demonstrated.

Understanding the genetic and epigenetic code of osteoporosis: Integrating the central role of Sirtuins in skeletal ageing

Hormone-sparing non-surgical male contraception in dogs and cats: Current strategies and the emerging role of FSH receptor-targeted antibody fragments.

Current non-surgical male contraceptive approaches in dogs and cats are summarized, including GnRH agonists, progestogens, androgens, systemic chemical contraceptive agents, intratesticular and intraepididymal chemical contraceptive agents, therapeutic ultrasonography, gene-editing strategies, and immunological approac...

Targeted allelic diversification of flowering genes via CRISPR-Cas enables the development of multiple early-flowering soybean lines

CRISPR-Cas multiplex editing generated a broad range of early-flowering soybean lines, advancing flowering by up to four weeks under controlled long-day conditions and by approximately one to two weeks in a field trial. Soybean is a promising crop for European agriculture, but its cultivation area remains smaller than...

Harnessing next-generation molecular technologies for precision control of Tuta absoluta and Helicoverpa armigera in tomato

Advanced molecular approaches providing sustainable resistance against insect pests have been discussed, like transgenic expression of insecticidal proteins, RNAi (RNA interference) strategies including HIGS and SIGS, genome editing by CRISPR-Cas, protease inhibitors and male sterility.

A ΦKMV ligase-dependent DNA repair mechanism that mitigates DNA-targeting nucleases

A broad-acting mechanism that allows Phikmvvirus phages to evade nuclease targeting is described and phage escape through faithful repair is described and a potentially interesting gene for phage therapy is identified.

Micronutrient Dynamics in Purple Cauliflower: Physiological Roles, Molecular Regulation, Anthocyanin Accumulation and Future Perspectives - A Critical Review

A comprehensive synthesis of micronutrient dynamics in purple cauliflower is provided and advanced biofortification models, nano-fertilizer integration, CRISPR/Cas gene editing, AI-assisted precision nutrition and multi-omics frameworks are critically evaluated to establish future strategies for climate-resilient and n...

Bigger than CRISPR? A guide to the latest genome editors

A computer generated molecular structure of the CRISPR-Cas9 gene editing complex is shown, showing the guide RNA is blue, and the double-strand of the DNA is green.

Molecular design and mechanistic understanding of lipo-xenopeptide carriers for nucleic acid delivery

Initially, viral vectors represent the most efficient systems in terms of nucleic acid delivery due to their inherent evolutionary adaption for cellular entry and genomic delivery (17,18).Nonetheless, increasing interest has resulted in the development of synthetic, non-viral delivery systems as more modulable and adap...

A Comprehensive Review on Stem Cell Therapy and CRISPR/Cas Technology in the Treatment of Thalassemia

A variety of research is synthesized, examining the therapeutic potential of stem cell therapy and CRISPR/Cas technology in the treatment of thalassemia, highlighting the use of stem cell therapy and CRISPR/Cas gene editing technology as alternative treatment options.

CanProTarget

A residue-resolved prioritization platform for covalent cancer drug discovery. CanProTarget integrates cancer gene dependencies, small-molecule covalent ligand engagement measured by chemoproteomics, and base-editing evidence for individual cysteines, keeping each layer at the resolution it is measured at.