gene editing

Comparison of Prime Editing and CRISPR-Cas Mediated HDR in Correcting CFTR-F508del Mutation in Patient iPSC-Derived Airway Basal Cells

Gene editing therapy represents a promising strategy to permanently cure cystic fibrosis (CF). This paper presents the results of correcting the most common CF mutation, F508del, in the CFTR gene in airway basal cells (BCs) obtained from patients, using both classical CRISPR-Cas9-mediated homology-directed repair (HDR)...

Integrated Transcriptomic and Metabolomic Analysis of OsMRS2-6 in Rice Growth and Chloroplast Traits

Background/Objectives: OsMRS2-6 is a member of the MRS2/MGT family, but its relationship with chloroplast status and growth and development in rice remains incompletely understood. Methods: In this study, we generated OsMRS2-6 knockout and overexpression lines and combined transient subcellular localization, chloroplas...

Meta-Analysis of Porcine Beige Adipogenesis with Cross-Species CD137-CD137L Molecular Docking: Potential Implications for Pig-to-Human Xenotransplantation

The findings support a hypothesis that porcine adipocyte beiging and CD137-related cross-species interactions may be relevant to xenograft immunometabolism; direct experimental validation in xenotransplantation models is required.

Coupling a developmental promoter to CRISPR interference for Wnt pathway regulation in human pluripotent stem cells

While directed differentiation of human pluripotent stem cells commonly relies on the timed delivery of extracellular factors, these uniform treatments often yield heterogeneous responses across cell populations. Linking intracellular gene regulation directly to an emerging developmental state offers a complementary st...

Alfalfa-mediated phytoremediation and soil restoration for sustainable cereal and fruit crop production: a review of roles, detoxification mechanisms, nitrogen enhancement, and agroecological integration

Soil contamination by heavy metals, petroleum hydrocarbons, and excessive agrochemical inputs, coupled with declining soil fertility, poses a major threat to sustainable cereal and fruit production and global food security. This review synthesizes evidence from peer-reviewed studies published between 2000 and 2026 to...

Waterlogging tolerance in pigeonpea: current understanding of physiological responses, genetic resources, and genomic prospects

An evidence-based framework provides a roadmap for moving from observed tolerance mechanisms toward experimentally validated molecular targets and their deployment in climate-resilient pigeonpea breeding.

Pathway-wide base editing charts chemical-genetic interactions in MAPK signaling

CRISPR base editor (BE) scanning enables sequence-level interrogation of proteins at scale in their native cellular and genomic contexts. This approach opens new opportunities to dissect cellular pathways, where signaling depends on coordinated interactions among multiple pathway proteins. We apply BE scanning to the R...

Therapeutic upregulation of gene expression in inherited cardiomyopathies from current approaches to future directions.

Defending synthetic DNA orders against intra-order splitting-based obfuscation

Biosecurity screening of synthetic DNA orders is a key defense against malicious actors and careless enthusiasts producing dangerous pathogens or toxins. It is important to evaluate biosecurity screening tools for potential vulnerabilities and to work responsibly with providers to ensure that vulnerabilities can be pat...

Advances in plant metabolic engineering through synthetic biology

This review highlights the primary technologies used in plant metabolic engineering and focuses on representative examples of their implementation for crop improvement, including but not limited to CRISPR-Cas systems, multiplex genome editing, knock-in and knock-out strategies, CRISPR-mediated gene regulation, base edi...

Phytomedicinal approaches to postprandial hyperglycemia: Plants with α‑amylase and α‑glucosidase inhibitory effects (Review)

Diabetes mellitus (DM), a non-communicable disease with a high disease burden, is characterized by insulin resistance and postprandial hyperglycemia.An estimated 589 million adults worldwide were suffering from DM in 2024, a number projected to exceed 853 million by the year 2050.Diabetes has an evolving epidemiologica...

Wheat genetic resources for sodicity tolerance: from salinity-derived evidence toward sodicity-specific breeding