Aug 2026· Sheng wu gong cheng xue bao = Chinese journal of biotechnology· Vol 42 8, pp.
3736-3748
· 0 citations
Medicine
TL;DR
The generation of a WSL-Cas9 monoclonal cell line with high CRISPR/Cas9 editing efficiency, stable proliferation, and permissiveness for robust ASFV replication is reported, providing a reliable platform for future genome-wide functional screening to systematically identify host factors governing ASFV entry.
This study successfully identified CX3CL1 as a potential negative regulator of TLS formation in bladder cancer through CRISPR-Cas9 library screening combined with in vivo validation, and provides new clues and research directions for understanding the molecular mechanisms of TLS formation.
Yongcun Wang, Hong-Cheng Song, Yi-Qing Du et al.· Beijing da xue xue bao. Yi x...· 0 citations
The results demonstrate that transcriptomics-guided host-cell engineering is an effective strategy to identify and relieve intrinsic bottlenecks in stable LVV producer cell lines.
Jona Röscheise, Lena-Marie Eberle, Holger Laux et al.· Biotechnology and Bioenginee...· 0 citations
An industrial-grade platform based on monoclonal producer cell lines that enables the continuous and scalable generation of engineered virus-like particles (eVLPs) co-packaging Cas9–gRNA ribonucleoproteins (RNPs) and provides a GMP-compliant and broadly adaptable strategy for the streamlined manufacturing of next-gener...
Wei Lin, Jia-Ru Shi, Hanyi Chen et al.· Frontiers in Immunology· 0 citations
This study establishes a reverse genetics platform for an emerging G2c PEDV strain and provides a stable fluorescent reporter virus, offering valuable tools for visualizing viral infection and investigating virus–host interactions.
Fan Zhang, He-Lu Liu, Linlong Ji et al.· Viruses· 0 citations
Cas9 ablation enabled restoration of CUL4B expression and function following introduction of a CUL4B expression plasmid, providing proof of concept for a broadly applicable approach to studying protein function through rescue experiments.
Lauren Postell, Nader Elturk, Hannah Leonard et al.· Journal of Visualized Experi...· 0 citations
It is reported that the choice of sgRNA target site affects its targeted cleavage activity and show that the combination of guideRNA with SpCas9 can effectively delete the exonic fragment of the SLC25A38 gene in HEK293T cells.
S. Yazdanparast, Hamid Galehdari, S. Khatami et al.· Journal of Applied Genetics· 0 citations
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