Jul 2026· International Journal of Research in Pharmacology & Pharmacotherapeutics· Vol 15, pp. 1129-1151· 0 citations
TL;DR
It is demonstrated that the 50% methanolic extract of Curcuma pseudo-montana rhizomes possesses significant hepatoprotective activity against D-galactosamine-induced liver injury, supporting the traditional use of the plant in liver disorders.
Abstract
Background: Liver diseases remain a major global health problem, and currently available therapies are often associated with limitations and adverse effects. Medicinal plants rich in antioxidant phytoconstituents have emerged as promising alternatives for the management of hepatic disorders. Curcuma pseudo-montana is traditionally used in folk medicine for the treatment of various ailments, including liver-related disorders.
Objective: The present study was undertaken to evaluate the hepatoprotective effect of 50% methanolic extract of Curcuma pseudo-montana rhizomes against D-galactosamine-induced hepatic injury in rats.
Materials and Methods: Hepatic injury was induced in Wistar albino rats using D-galactosamine (600 mg/kg, i.p.). Animals were treated with 50% methanolic extract of Curcuma pseudo-montana rhizomes at doses of 200 and 400 mg/kg body weight. Silymarin (100 mg/kg) was used as the standard hepatoprotective drug. Serum biochemical markers including SGOT, SGPT, ALP, bilirubin, and total protein were evaluated. Antioxidant parameters such as catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), and malondialdehyde (MDA) were estimated. Histopathological examination of liver tissues was also performed.
Results: D-galactosamine administration produced significant hepatic injury characterized by elevated serum SGOT, SGPT, ALP, and bilirubin levels along with a reduction in total protein content. Oxidative stress was evidenced by decreased CAT, SOD, and GSH levels and increased MDA concentration. Treatment with the 50% methanolic extract significantly restored liver function markers and antioxidant enzyme levels in a dose-dependent manner. The higher dose (400 mg/kg) demonstrated greater hepatoprotective activity and significantly reduced lipid peroxidation. Histopathological examination confirmed marked protection against hepatocellular degeneration, necrosis, and inflammatory infiltration, with near-normal hepatic architecture observed in the high-dose treatment group.
Conclusion: The findings demonstrate that the 50% methanolic extract of Curcuma pseudo-montana rhizomes possesses significant hepatoprotective activity against D-galactosamine-induced liver injury. The protective effect may be attributed to its antioxidant and anti-inflammatory properties, supporting the traditional use of the plant in liver disorders.
Pretreatment with PEIV significantly mitigated effects of LPS-induced liver injury by regulating biochemical parameters, reducing oxidative stress and inflammation, and preserving liver architecture, suggesting its potential as a natural therapeutic agent for liver diseases.
S. Anadozie, A³ Maryam, Theophilus I. Ebe et al.· Comparative Clinical Patholo...· 0 citations
Findings suggest that the hepatoprotective effect of HAEIB may be attributed to its antioxidant phytoconstituents and support its potential use as a natural hepatoprotective agent.
Shweta Patil, A. Sachin, Pratiksha Vadhane et al.· Genetics and Molecular Resea...· 0 citations
The estimated biomarkers provide evidence that the Lannea coromandelica bark extract has shown hepatoprotective activity against paracetamol induced hepatotoxicity in rats.
Md Rashed Hossen, Khaleda Nahid, M. Rahman et al.· Bangladesh Pharmaceutical Jo...· 0 citations
Under the experimental conditions, the individual ethanolic extracts, particularly O. gratissimum, were associated with improved hepatorenal biochemical indices, whereas combined administration was associated with marked hepatic.
C. G. Ehie, Ahamefula Sunday Ezekwe, A. Okari et al.· Health Science Research Inte...· 0 citations
Background: Drug-induced liver injury (DILI) is a major cause of hepatic morbidity. Isoniazid (INH), a first-line
antitubercular drug, is frequently associated with hepatotoxicity due to the formation of reactive metabolites that
deplete hepatic antioxidant defenses. Plant-derived antioxidants may offer protection against such injury.
Objective: To evaluate the hepatoprotective potential of the ethanolic seed extract of Coix lacryma-jobi L. (CLJSE)
against isoniazid-induced hepatotoxicity using in vitro and in vivo models.
Methods: CLJSE was prepared by Soxhlet extraction using 70% ethanol and standardized through pharmacognostic,
physicochemical, phytochemical, and FTIR analyses. Antioxidant activity was assessed by the DPPH assay, while
hepatoprotective activity was evaluated in HepG2 cells against H₂O₂-induced cytotoxicity using the MTT assay. Acute
oral toxicity was conducted according to OECD Guideline 423. For the in vivo study, Wistar rats were divided into
normal control, toxic control (INH, 50 mg/kg i.p., twice weekly for 28 days), standard (INH + silymarin 100 mg/kg
p.o.), and extract-treated groups (INH + CLJSE 200 or 400 mg/kg p.o.). Serum liver markers, hepatic antioxidant
parameters, liver weight, and histopathology were evaluated.
Results: CLJSE showed concentration-dependent DPPH radical scavenging activity (IC₅₀ = 74.56 µg/mL) and
protected HepG2 cells against oxidative stress, producing 94.46% cell viability at 100 µg/mL. The extract was nontoxic up to 2000 mg/kg. INH significantly increased serum AST, ALT, ALP, total bilirubin, liver weight, and MDA
levels, while reducing GSH, SOD, and CAT levels (p < 0.01). CLJSE treatment significantly and dose-dependently
reversed these changes, with the 400 mg/kg dose showing effects comparable to silymarin. Histopathological findings
confirmed marked protection of hepatic architecture.
Conclusion: CLJSE exhibits significant dose-dependent hepatoprotective activity against isoniazid-induced liver
injury, primarily through antioxidant, anti-lipid-peroxidative, and membrane-stabilizing mechanisms, supporting its
potential as an adjuvant phytotherapeutic agent during antitubercular therapy.
Soniya C. Deshmukh, K. Aghade, Nitin P. Jain et al.· International Journal of Dru...· 0 citations
Liver diseases are a major global health burden, and oxidative stress plays
a key role in the development of hepatocellular injury. Mimusops elengi L. fruits contain bioactive
phytochemicals with reported antioxidant properties. This study aimed to evaluate the hepatoprotective
activity of aqueous Mimusops elengi fruit extract against carbon tetrachloride (CCl4)-induced
liver injury in Wistar rats.
Male Wistar rats were randomly allocated into five groups (n = 5). Hepatic injury was
induced using CCl4 (1 mL/kg, i.p.) for 15 days. Animals received Mimusops elengi fruit extract (200
and 400 mg/kg, orally) or silymarin (25 mg/kg). Serum ALT, AST, and catalase activity were evaluated,
followed by histopathological examination of liver tissues.
CCl4 administration significantly increased serum ALT and AST levels while reducing catalase
activity, indicating severe hepatic injury. Treatment with Mimusops elengi fruit extract significantly
reduced liver enzyme levels and improved antioxidant status in a dose-dependent manner.
Histopathological examination demonstrated preservation of hepatic architecture, reduced necrosis
and decreased inflammatory changes, particularly at 400 mg/kg.
The hepatopr tective activity observed may be attributed to the antioxidant and membrane-
stabilizing properties of flavonoids, tannins, and saponins present in Mimusops elengi fruits.
The biochemical and histopathological findings collectively support the traditional medicinal use of
this plant for liver protection and suggest its potential as a natural hepatoprotective agent.
Mimusops elengi fruit extract exhibits significant hepatoprotective effects against
CCl4-induced liver injury, likely mediated through antioxidant mechanisms. These findings support
its potential as a natural therapeutic agent, although further studies are required to elucidate its active
constituents and molecular pathways.
Nisha Chandila, Suresh Kumar, Manoj Kashyap· The International Journal of...· 0 citations
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