EVALUATION OF THE HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITIES OF HYDROALCOHOLIC EXTRACT OF IMPATIENS BALSAMINA LINN. LEAVES AGAINST DICLOFENAC-INDUCED HEPATOTOXICITY IN RATS
Aug 2026· Genetics and Molecular Research· 0 citations· 12 references
TL;DR
Findings suggest that the hepatoprotective effect of HAEIB may be attributed to its antioxidant phytoconstituents and support its potential use as a natural hepatoprotective agent.
Abstract
Drug-induced liver injury is a major health concern and represents one of the most common causes of acute liver failure. Diclofenac, a widely prescribed non-steroidal anti-inflammatory drug (NSAID), is known to induce hepatotoxicity through oxidative stress, mitochondrial dysfunction, and the formation of reactive metabolites. Natural products with antioxidant properties have gained attention as potential hepatoprotective agents. Impatiens balsamina Linn. is a medicinal plant traditionally used for various therapeutic purposes and is rich in flavonoids, phenolic compounds, tannins, and other bioactive constituents with potent antioxidant activity. The present study was undertaken to evaluate the hepatoprotective activity of the hydroalcoholic extract of Impatiens balsamina leaves (HAEIB) against diclofenac-induced hepatotoxicity in experimental rats. HAEIB treatment significantly restored altered biochemical parameters, increased GSH and SOD levels, and reduced LPO levels compared with the diclofenac-treated group, indicating attenuation of oxidative stress. Histopathological examination revealed marked protection against hepatic degeneration, necrosis, and inflammatory cell infiltration. HAEIB at a dose of 400 mg/kg demonstrated hepatoprotective activity comparable to that of the standard drug silymarin. These findings suggest that the hepatoprotective effect of HAEIB may be attributed to its antioxidant phytoconstituents and support its potential use as a natural hepatoprotective agent.
Findings demonstrate that the alcoholic extract of Panax ginseng possesses both preventive and therapeutic hepatoprotective properties against diclofenac-induced liver injury, likely mediated through its antioxidant and anti-inflammatory mechanisms.
Sami Ahmed· Kufa Journal For Veterinary...· 0 citations
Pretreatment with PEIV significantly mitigated effects of LPS-induced liver injury by regulating biochemical parameters, reducing oxidative stress and inflammation, and preserving liver architecture, suggesting its potential as a natural therapeutic agent for liver diseases.
S. Anadozie, A³ Maryam, Theophilus I. Ebe et al.· Comparative Clinical Patholo...· 0 citations
Background: Drug-induced liver injury (DILI) is a major cause of hepatic morbidity. Isoniazid (INH), a first-line
antitubercular drug, is frequently associated with hepatotoxicity due to the formation of reactive metabolites that
deplete hepatic antioxidant defenses. Plant-derived antioxidants may offer protection against such injury.
Objective: To evaluate the hepatoprotective potential of the ethanolic seed extract of Coix lacryma-jobi L. (CLJSE)
against isoniazid-induced hepatotoxicity using in vitro and in vivo models.
Methods: CLJSE was prepared by Soxhlet extraction using 70% ethanol and standardized through pharmacognostic,
physicochemical, phytochemical, and FTIR analyses. Antioxidant activity was assessed by the DPPH assay, while
hepatoprotective activity was evaluated in HepG2 cells against H₂O₂-induced cytotoxicity using the MTT assay. Acute
oral toxicity was conducted according to OECD Guideline 423. For the in vivo study, Wistar rats were divided into
normal control, toxic control (INH, 50 mg/kg i.p., twice weekly for 28 days), standard (INH + silymarin 100 mg/kg
p.o.), and extract-treated groups (INH + CLJSE 200 or 400 mg/kg p.o.). Serum liver markers, hepatic antioxidant
parameters, liver weight, and histopathology were evaluated.
Results: CLJSE showed concentration-dependent DPPH radical scavenging activity (IC₅₀ = 74.56 µg/mL) and
protected HepG2 cells against oxidative stress, producing 94.46% cell viability at 100 µg/mL. The extract was nontoxic up to 2000 mg/kg. INH significantly increased serum AST, ALT, ALP, total bilirubin, liver weight, and MDA
levels, while reducing GSH, SOD, and CAT levels (p < 0.01). CLJSE treatment significantly and dose-dependently
reversed these changes, with the 400 mg/kg dose showing effects comparable to silymarin. Histopathological findings
confirmed marked protection of hepatic architecture.
Conclusion: CLJSE exhibits significant dose-dependent hepatoprotective activity against isoniazid-induced liver
injury, primarily through antioxidant, anti-lipid-peroxidative, and membrane-stabilizing mechanisms, supporting its
potential as an adjuvant phytotherapeutic agent during antitubercular therapy.
Soniya C. Deshmukh, K. Aghade, Nitin P. Jain et al.· International Journal of Dru...· 0 citations
The present study aim that Alternanthera brasiliana (AB) possesses significant hepatoprotective, antioxidant, and anti-inflammatory properties against CCl₄-induced liver injury. Phytochemical analysis revealed that AB extracts, particularly the hydroalcoholic and chloroform fractions, are rich in phenolics, flavonoids, terpenoids, and alkaloids, many of which are known for their bioactivity. In-vitro assays confirmed strong free radical scavenging and reducing power, consistent with the phytochemical profile. These findings suggest that the hepatoprotective efficacy of Alternanthera brasiliana is mediated through a synergistic interplay of antioxidant, anti-inflammatory, and antifibrotic mechanisms. The results highlight AB as a promising candidate for development as a natural hepatoprotective agent or adjunctive therapy in managing liver disorders associated with oxidative stress and inflammation
Mandapelly Srivani, V. Umarani, D. Swathi et al.· International journal of che...· 0 citations
It is indicated that combining papaya, neem, and garlic extracts may provide greater hepatoprotection than administering the individual extracts against DEN-induced liver injury, and supports further mechanistic and preclinical evaluation of this polyherbal formulation before its potential therapeutic application in liver-disease management.
Egeonu Us, Umaru I. J., Izam Ed et al.· African Journal of Clinical...· 0 citations
Thunbergia laurifolia has long been used in traditional medicine for detoxification; however, the biological basis of its detoxifying effects remains poorly understood. This study investigated the detoxifying effects of a phenolic-rich aqueous extract of T. laurifolia using integrated in vitro and in vivo approaches. Caffeic acid (CA) and rosmarinic acid (RA) were used as marker compounds for extract standardization. The aqueous extract was evaluated for antioxidant, acetylcholinesterase, and apoptosis-modulating activities in vitro, followed by evaluation in a chlorpyrifos-induced oxidative stress model in vivo. The extract exhibited antioxidant activity and maintained acetylcholinesterase (AChE) activity. In LX-2 hepatic stellate cells, the extract induced predominantly early apoptosis, whereas CA and RA induced predominantly late apoptosis. In chlorpyrifos-exposed rats, the extract restored AChE activity, reduced lipid peroxidation, and enhanced endogenous antioxidant defenses, as evidenced by decreased malondialdehyde (MDA) levels and increased glutathione (GSH) levels and superoxide dismutase (SOD) activity. Collectively, these findings suggest that attenuation of oxidative stress may represent one of the mechanisms underlying the detoxifying effects traditionally attributed to T. laurifolia.
P. Kunnaja, S. Intatham, P. Khonsung et al.· International Journal of Mol...· 0 citations
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