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Transferable genetic toolsets for nonmodel gut Clostridia enable in vivo reversible control of metabolite production.

Ting-Ting Li Xi Chen Fang Wang Yuelin Angelina Tang M. Sim Leyi Xiao Wen-Bing Jin Huiqing Shi Jia-Yan Ma Xiaoyu Yang Yaping Liu Matthew T. Sorbara Chun-Jun Guo
Aug 2026 · Nature Biotechnology · 0 citations · 77 references
Medicine

TL;DR

This work identifies a panel of strong constitutive promoters that drive robust gene expression across diverse clostridial strains and develops an inducible promoter system that enables precise, tunable gene regulation and facilitates the implementation of CRISPR-Cas gene-deletion systems.

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