· Methods in molecular biology· Vol 3074, pp.
193-208
· 0 citations
Medicine
TL;DR
An ECCITE-seq framework is described to characterize the transcriptomic consequences of perturbing multiple neuronal key driver genes associated with Alzheimer's disease (AD) in human-induced pluripotent stem cell (hiPSC)-derived neurons and allows for the assessment of the regulatory impact of candidate genes implicated in development and disease processes.
Perturb-ME, along with agentic interpretation, provide a scalable framework for comprehensive functional discovery from phenotype-enriched genetic screens and combines genome-scale CRISPR screening, phenotype-based enrichment and multimodal single-cell profiling.
Han-Chen Wang, Jiacheng Gu, Chris J. Frangieh et al.· bioRxiv· 0 citations
It is demonstrated that integrated Perturb-seq experiments spanning diverse contexts enable hypotheses about gene function specific to tissue types or cancer subtypes – suggesting large-scale, genome-wide datasets would offer invaluable insight into the highly context-dependent nature of cancer biology.
Samuel Maffa, Isabella A. Boyle, Lie Ward et al.· bioRxiv· 0 citations
A scalable, semi-automated, and physiologically relevant hiPSC-derived macrophage model, rigorously characterised through deep comparative multi-omics is established and how genetic perturbations alter pro- and anti-inflammatory transcriptional signatures and significantly impact functional phenotypes are showcased.
A scalable, cell-type-resolved in vivo CRISPR interference (CRISPRi) platform enabling systematic gene function profiling in the mouse brain is developed and the CRISPRinvivo data portal is established as a community resource for in vivo screening.
Risheng Lin, Ze-Ting Ke, Jian-Hui Wang et al.· Neuron· 0 citations
Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 screening has become a central technology in functional genomics, enabling genome‐scale interrogation via pooled perturbations. Early CRISPR screens employed survival or simple phenotypic readouts to identify essential genes and drug resistance mec...
SCITO-Perturb-seq represents the first genome-wide CRISPRa screen paired with direct, high-dimensional surface protein profiling, providing a comprehensive regulatory map of the CD4 T cell surface proteome.
Yutong V. Wang, Junha Park, Min Cheol Kim et al.· bioRxiv· 0 citations
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