protein folding

ArcRefine improves de novo binder designs while preserving binding modes

De novo protein design now enables the creation of high-affinity binders from a target structure alone. However, current design methods face a trade-off between site control and success rate. Generative models such as RFdiffusion and BoltzGen place binders at chosen sites but often require large candidate pools. Halluc...

STRUCTURE OF A THERMOSTABLE DISULFIDE-BRIDGE MUTANT OF PHAGE T4 LYSOZYME SHOWS THAT AN ENGINEERED CROSSLINK IN A FLEXIBLE REGION DOES NOT INCREASE THE RIGIDITY OF THE FOLDED PROTEIN

A conserved achaete-scute cluster in three tardigrade genomes, invisible to all three annotations

The achaete-scute complex is one of the best-studied gene clusters in animal development and nobody has looked for it in a tardigrade: PubMed returns no records for achaete-scute combined with tardigrad*, Hypsibius or panarthropod*. We searched the sequences rather than the annotations. An HMM search with the curated H...

Temporary Cessation of DMARD Therapy and COVID-19 Supplemental Dose Vaccine Immunogenicity in Patients With Inflammatory Arthritis: The COVER Randomized Clinical Trial.

Importance The COVID-19 pandemic disproportionately affected patients with autoimmune and rheumatic diseases. Understanding whether certain medications affect the response to a SARS-CoV-2 supplemental dose vaccination is critical for counseling and treating patients receiving targeted immunomodulatory medications. The...

Bispecific Coiled-Coil Reporters Enable Rapid, Multiplexed Detection of Mycoplasma via Toehold Switch Sensors

Abstract Cell-free synthetic biology offers a rapid prototyping environment for molecular diagnostics, yet the utility of these systems is often limited by the slow kinetics and restricted multiplexing capabilities of traditional protein reporters. Here, we report the BINOCULAR (BIspecific Novel Orthogonal Coiled-coil-...

A conserved achaete-scute cluster in three tardigrade genomes, invisible to all three annotations

The achaete-scute complex is one of the best-studied gene clusters in animal development and nobody has looked for it in a tardigrade: PubMed returns no records for achaete-scute combined with tardigrad*, Hypsibius or panarthropod*. We searched the sequences rather than the annotations. An HMM search with the curated H...

Most predicted drug pockets in the apicomplexan dark proteome are artefacts of non-globular structure.

Structural genomics is used to find drug targets among the proteins a genome leaves unnamed: a protein with no sequence homologue but a confidently predicted fold looks both novel and tractable. I show that in Plasmodium falciparum this reasoning fails four times before it reaches a target list. Of 1017 confidently mod...

Thioredoxin h1-targeted proteins in European beech (Fagus sylvatica L.): Insights into metabolic control, stress response, and seed longevity

Seed viability and longevity underpin forest regeneration under changing environmental conditions. European beech (Fagus sylvatica L.) produces intermediate seeds that gradually lose viability during storage, largely through oxidative stress, which makes redox homeostasis central to their longevity. Here we investigate...

A sensitive colorimetric ELISA to measure allergen-specific salivary SIgA for monitoring sublingual immunity efficiency in pollinosis SLIT

Sublingual immunotherapy (SLIT) is safe and one of the most effective etiological treatment for pollinosis. Allergen-specific salivary secretory immunoglobulin A (SIgA) is a useful marker to objectively evaluate treatment efficacy. However, saliva samples usually exhibit high background noise because of non-specifi...

Figure 1 from BET Inhibition Synergizes with RLR Signaling to Enhance Tumor Immunogenicity and T-cell Recognition

BETi pretreatment sensitizes tumor cells to pIC-induced inflammation. A, Experimental setup for BETi pretreatment and pIC transfection of tumor cells. B, Protein abundance of phosphorylated IRF3 and (C) TBK1 in A375 cells after 72 hours of JQ1 or DMSO pretreatment and pIC transfection for 24 hours assessed by Western b...

Long Non-Coding RNA LINC01614 Promotes Aggressive Phenotypes and Ferroptosis Resistance in Papillary Thyroid Cancer Through a Putative miR-4521/IGF2/PI3K-AKT Signaling Axis

Background/Objectives: Papillary thyroid cancer (PTC) is the most common endocrine malignancy and is generally associated with favorable clinical outcomes. However, a subset of tumors develops aggressive phenotypes characterized by increased proliferation, migration, therapeutic resistance, and disease progression. Lon...

Icosahedral Symmetry and Quasi-Equivalence: The Triangulation Number Determines Viral Capsid Architecture — E8 Intelligence Research

FINDING: Viral capsid architecture is governed by icosahedral symmetry and quasi-equivalence theory, where the triangulation number T = h² + hk + k² (h,k ≥ 0 integers) determines the number of protein subunits (60T) and their arrangement on a spherical lattice. | MATH: T = h² + hk + k²; capsid protein count = 60T; allo...