Chinese hamster ovary (CHO) cells are widely used for recombinant therapeutic protein production. MicroRNA engineering represents a potential strategy for improving CHO cell performance; however, conventional random integration can introduce variability in transgene expression. Here, we investigated targeted miR-17 int...
Two public developmental transcriptomes of Hypsibius exemplaris, Levin et al. 2016's 62 single timed embryos and Yoshida et al. 2017's egg-to-adult series, have never been asked when a tardigrade transcribes its synaptic machinery: neither paper, nor two later reanalyses, mentions synapsin, synaptotagmin, VAChT, GABA o...
Meow-Ludo Meow-Meow· Zenodo (CERN European Organi...· 0 citations
Bone infections, including osteomyelitis and periprosthetic joint infection (PJI), are characterized by prolonged treatment, high recurrence, and functional impairment. Persistent inflammation induces hypercatabolism, negative nitrogen balance, and micronutrient depletion, impairing immune defense and bone repair....
Xiao-Qin Li, Can Zhang, Wei-Zhu Zhang et al.· Frontiers in Nutrition· 0 citations
Two public developmental transcriptomes of Hypsibius exemplaris, Levin et al. 2016's 62 single timed embryos and Yoshida et al. 2017's egg-to-adult series, have never been asked when a tardigrade transcribes its synaptic machinery: neither paper, nor two later reanalyses, mentions synapsin, synaptotagmin, VAChT, GABA o...
Meow-Ludo Meow-Meow· Zenodo (CERN European Organi...· 0 citations
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De novo protein design now enables the creation of high-affinity binders from a target structure alone. However, current design methods face a trade-off between site control and success rate. Generative models such as RFdiffusion and BoltzGen place binders at chosen sites but often require large candidate pools. Halluc...
Ken Osumi· Zenodo (CERN European Organi...· 0 citations
Coronary artery lesions (CAL) represent the most severe complication of Kawasaki disease (KD). This study aims to analyze the relationship between serum Interleukin-6 (IL-6) levels and CAL in KD, and develop a predictive model to provide a scientific basis for clinical risk assessment and personalized treatment of...
SRP54, a key component of the signal recognition particle, is a highly conserved enzyme both structurally and functionally. The protein plays a vital role in cellular protein translocation by interacting with the translating ribosome and a membrane receptor, thereby aiding protein folding and trafficking. Recent stud...
Ming Yang, Andrew D. Brim, Lawton F. Long et al.· ACS Omega· 0 citations
The Chikungunya virus (CHIKV) infection induces an acute illness and ongoing inflammatory symptoms, with macrophages believed to play significant yet not fully understood roles. To characterize host cellular responses associated with CHIKV infection, we performed mass spectrometry–based phosphoprotein enrichment-ba...
Most proteins start to fold cotranslationally as they come off the ribosome. So far, studies of cotranslational folding have focused mainly on small, single-domain proteins. Here, we have used Force Profile Analysis to study the cotranslational folding of Firefly Luciferase, a complex 550-residue protein composed of an...
Spyridoula Mitsikosta, Justin M. Westerfield, Fátima Pardo-Avila et al.· Nature Communications· 0 citations
Since 1986, replicate lines of the seed beetle Acanthoscelides obtectus have been kept under two experimental evolution regimes: Early (E; lines EI–EIV), where beetles reproduce only during the first two days of adult life, and Late (L; lines LV–LVIII), where egg laying is only possible after day 10. After over 200 gen...
Athanasios Toros, Mats E. Pettersson, Angela P. Fuentes‐Pardo et al.· SciLifeLab Data Repository· 0 citations
Mitochondrial DNA (mtDNA) base editors (mtBEs) are promising tools for studying and correcting pathogenic mtDNA mutations. However, the application of mtBEs is often hindered by substantial mtDNA-wide off-target editing that accumulates with prolonged mtBE expression resulting from plasmid DNA or viral delivery. To ove...
Hyewon Jang, Carolline Rodrigues Menezes, Krzysztof Palczewski et al.· bioRxiv (Cold Spring Harbor...· 0 citations
De novo protein design now enables the creation of high-affinity binders from a target structure alone. However, current design methods face a trade-off between site control and success rate. Generative models such as RFdiffusion and BoltzGen place binders at chosen sites but often require large candidate pools. Halluc...
Ken Osumi· Zenodo (CERN European Organi...· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
