This study establishes the feasibility of dCasRx-mediated programmable RNA base editing in plant cells and lays a foundation for further optimization of editing efficiency and stable in planta editing performance.
Xue-Ming Ruan, Zi-Yan Zhang, Xiang-Yu Xiong et al.· Biochemical and Biophysical...· 0 citations
This ex vivo primary ferret nasal epithelial cell and peripheral blood mononuclear cell co-culture model shows that signaling decoupling across the "epithelial-immune bridge" drives suboptimal IBV immunogenicity.
This first study to include Olink Reveal in a systematic cross-platform comparison and to directly compare the Orbitrap Astral with the previous-generation Orbitrap Exploris 480 under identical conditions provides a concrete, evidence-based framework for workflow selection and support the co-deployment of deep MS and h...
Jean-Marc Monneuse, Hayat Hage, Célie Da Silva et al.· Journal of Proteomics· 0 citations
Results demonstrated that ProtThermoRank provides a sequence-based approach for artificial intelligence-guided protein engineering, enabling in-silico high-throughput screening of thermostabilizing variants and accelerating the discovery and optimization of highly stabilizing proteins.
Sungbo Hwang, Sohee Oh, Jin-Sik Kim et al.· International Journal of Bio...· 0 citations
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The results demonstrate the promising potential of PCV2 VLPs as versatile epitope-display platforms, while establishing the IDR as a suitable fusion site that maintains both structural integrity and biological functionality.
Hong Li, Xiao-Ming Huang, Yu-Xin Zhao et al.· International Journal of Bio...· 0 citations
Osteogenesis imperfecta (OI) is a genetically heterogeneous connective tissue disorder characterized by bone fragility, recurrent fractures, skeletal deformities, and substantial variability in clinical severity. Although OI is primarily associated with defects in type I collagen or proteins involved in its processing,...
Zhiming Wu, Regenerative Medicine and Stem Cells, ORT Research et al.· Utrecht University Repositor...· 0 citations
Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, yet the molecular mechanisms driving tumor initiation and progression are incompletely understood. Here, we integrate single-cell long-read sequencing, proteomics, and DNA methylation data to identify tumor-specific targets from unan...
Background Fibromyalgia (FM) is a disorder characterized by chronic pain and somatic symptoms that are not fully understood. Central sensitization, a process by which the central nervous system amplifies pain signals, is a key aspect of the pathophysiology of fibromyalgia. Despite its prevalence, effective treatment op...
Mammalian gene switches enable programmable cell behavior. However, current switches on transcriptional and translational layers require de novo RNA and protein synthesis, and secreted outputs must additionally undergo folding, post-translational processing, and intracellular trafficking, imposing delays that limit rap...
H-10 elicited dose-dependent seizure suppression and alleviated multi-organ pathological damage in both acute and chronic epileptic mouse models and is an orally bioavailable, efficacious GABAAR-targeted PAM with broad-spectrum antiseizure activity, deserving further optimization for anticonvulsant drug discovery.
Di Hu, Ying Wang, Jin-Ling Yu et al.· European journal of medicina...· 0 citations
ESMCHalo is a frozen protein-sequence predictor that combines ESMC-600M layer-36 canonical mean representations with five compact student heads trained using out-of-fold teacher targets with documented cross-fold limitations. The release provides frozen inference code, model checkpoints, calibration, a fixed threshold,...
Fang Lv, Hutao Jin, Qishuai Ma et al.· Zenodo (CERN European Organi...· 0 citations
Particle geometry can alter nanomedicine transport, yet its intrinsic contribution is difficult to define because shape engineering usually changes particle composition or surface chemistry. Here we use sonication to transform compositionally identical SN38 dimeric prodrug nanoassemblies from spheres into rods with asp...
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
