protein folding

Multiple strategies to improve the yield of snake thrombin-like enzyme agkihpin from Gloydius halys pallas in Pichia pastoris

A multi-strategy approach in Pichia pastoris is developed to enhance the secretory expression of agkihpin, a snake venom thrombin-like enzyme that possesses thrombin-like activity and exhibits multiple functions, including inhibition of tumor invasion and metastasis.

APOA1 links a late fetal epithelial program to colonic maturation and injury responses

Developmental programs establish tissue identity and organization, but how their reactivation contributes to adult tissue responses remains poorly understood. Here, we identify a functional contribution of apolipoprotein A-I (APOA1), which marks a late fetal colonic epithelial state that reappears after injury. Spatial...

Duqueana Core - p53 Structural Restoration Analysis v1.0.0 Detección Determinista de Ángulo de Ruptura y Vector de Restauración

================================================================================ Duqueana Core - p53 Structural Restoration Analysis v1.0.0 Detección Determinista de Ángulo de Ruptura y Vector de Restauración ================================================================================ Autor: Douglas Helvesio Urbina...

Postprandial amino acid profiles and gastric behavior after consumption of milk and plant-based alternatives: a randomized cross-over study in healthy males

Background: There is a growing availability of plant-based alternatives for bovine milk. However, plant-based drinks differ e.g. in protein quantity, protein quality and carbohydrate composition. Knowledge on the impact this has on digestion-related outcomes such as postprandial amino acid (AA) availability and gastric...

Abstract B095: Discovery of treatment-associated peptide antigens for targeted immunotherapies in pancreatic ductal adenocarcinoma

This work provides a framework for identifying actionable treatment-induced peptide antigens and supports rational combination strategies pairing KRAS/RAS inhibitors with T cell-based immunotherapies, including BiTE, CAR-pMHC, TCR-T, and cancer vaccine approaches.

Artificial Biosynthetic Pathways for Cell-Free Production of Raspberry Ketone and the Cardiotonic Drug Dobutamine

Raspberry ketone (RK) is a high-value flavor and fragrance compound widely used in the food, beverage, and cosmetic industries. However, existing biosynthetic routes largely rely on native metabolic pathways that involve multiple enzymatic steps and exhibit limited production efficiency. Here, we designed and constru...

Class-specific cysteine folding sensitivity in protein language models: evidence from iron–sulfur and disulfide-rich proteins

Cross-Dataset Genomic Proximity and Functional Association of FOXA1 and TET2 at Hypomethylated Regulatory Regions in Colorectal Cancer

Background/Objectives: Aberrant DNA methylation plays a critical role in the development and progression of colorectal cancer (CRC). However, how pioneer transcription factors are associated with DNA demethylases at cis-regulatory elements and influence tumor-associated transcriptional programs remains poorly understoo...

Infection susceptibility and severity in the UK Biobank proteome

Infection susceptibility and severity in the UK Biobank proteome Analysis notebook accompanying "The Frailty Proteome Predicts Infection Admission,\\but Only Weakly Predicts Survival" (Jacobs). It reproduces every number in the manuscript from the inputs described below and writes all exports to ./infect_out/. What it...

Correction: Analysing open-source protein folding models for nanobody binding prediction

Synthesis and Biological Evaluation of Prazosin-Derived Antitrypanosomal Agents Putatively Targeting FAZ1

Abstract Current therapeutic interventions for Human African Trypanosomiasis (HAT) are severely limited by emerging drug resistance and profound systemic toxicity, necessitating the urgent development of novel chemotypes. Building upon our previous identification of the alpha-adrenoceptor antagonist Prazosin as a scaff...

Abstract A065: Mutant GNAS drives cAMP/PDE/AMP–pAMPK signaling and therapeutic synergy with combined CDK4/6 and RAS inhibition in mucinous pancreatic neoplasia

Intraductal Papillary Mucinous Neoplasm (IPMN) are pre-malignant lesions of the pancreas with frequent co-mutations in KRAS and GNAS oncogenes. We recently reported pancreatic neoplasia from both primary and metastatic disease with KRAS/GNAS mutations are sensitive to single agent treatment with the FDA approved cell...