protein folding

Influence of Actinomucor elegans Fermentation on Protein Degradation, Flavor Development, and Nutritional Quality of Tofu

Clinical features associated with macrophage activation syndrome in juvenile dermatomyositis: implications for early recognition

This study aimed to characterize the clinical and laboratory features associated with juvenile dermatomyositis-associated macrophage activation syndrome and to identify warning features that may facilitate recognition of concurrent MAS at JDM presentation. The distribution and potential discriminatory contribution of m...

Responses of the model crop plant Ocimum basilicum L. grown hydroponically to combined hypoxia and salinity

Basil ( Ocimum basilicum L., cv Genovese) cultivated under hydroponic conditions was subjected to salinity, hypoxia, and combined stress for varying lengths of time to assess growth, metabolic, and antioxidant responses. Analyses demonstrated that stress type was the primary factor influencing plant responses, with tre...

Multi-Chain Hamiltonian Replica Exchange Framework for Probing Transient Helices and Order-Disorder Transitions in Proteins

Simulations have emerged as a pillar in biophysics to understand behavior at the molecular scale, in particular for proteins such as intrinsically disordered proteins. These often show transient folding with long-lived states that are challenging to efficiently sample using conventional simulations. Here, we show that...

Peptide-level reanalysis finds a small cleavage shift but no major accessibility bias under osmotic stress

Environmental stress can alter protein folding, molecular interactions, aggregation state, and protease accessibility before proteomic sample preparation. I reanalyzed the publicly available *Yersinia pseudotuberculosis* TMT11plex proteomics data associated with Süer et al. (PNAS, 2026) to test whether the 0.5 M NaCl o...

The bacterial antiphage protein Tiamat is an ATPase and DNase whose homologs are found across the tree of life

Bacteria and archaea encode hundreds of antiviral defense systems, some with homologs found in eukaryotic innate immunity. GHL ATPases like plant MORCs and prokaryotic Tiamat defend against viruses and phage, though their architecture and biochemical activities have remained unclear. Searching thousands of proteomes, w...

Hydrophobic ion pairing as a rational surface engineering strategy for enhanced protein integration within lipid-based nanocarriers

Hydrophobic ion pairing is an established solubility engineering strategy that uses surfactants to modulate drug lipophilicity and facilitate encapsulation in lipid-based nanocarriers. Although promising for enhancing lipophilicity, its influence on protein stability, activity, and interactions with lipids remains uncl...

Deciphering the role of AIM2 in myelin damage and repair in a murine model of multiple sclerosis

Multiple sclerosis (MS) is an immune-mediated disease of the central nervous system (CNS) targeting the myelin sheath and oligodendrocytes. The destruction of either or both myelin sheath or oligodendroglia can lead to the loss of underlying neurones and functional decline in patients. Myelin regeneration is possible i...

Proteomics and metabolomics-based identification of novel treatment strategies in pancreatic ductal adenocarcinoma

Introduction: Pancreatic ductal adenocarcinoma (PDAC) has very low survival rates that have not changed in over 40 years. Delay in diagnosis and the development of drug-resistance limits the effectiveness of current therapies, making identification of novel early detection and combination treatment strategies, extremel...

Markers and mechanisms of pre-eclampsia in pregnancies complicated by diabetes

Pre-eclampsia complicates 2-8% of pregnancies worldwide. Women with diabetes have a 4-fold increased risk of pre-eclampsia compared to the general population. Mechanisms for pre-eclampsia development are not well understood; however, evidence indicates that an increased release of two anti-angiogenic factors, soluble f...

Studies of vacuolar iron transporter homologue from Plasmodium falciparum in a yeast model system

Malaria is a serious and often a fatal disease in humans, caused by a parasite commonly transmitted by the female Anopheles mosquito. Plasmodium falciparum is by far the deadliest of all malaria parasites. According to the most current World Malaria Report, there were 247 million cases of malaria in 2021, up from 245 m...

Structure-function studies of Borrelia metal transport A (BmtA) from Lyme disease-causing Borrelia burgdorferi

Lyme disease, caused by the spirochetal bacterium Borrelia burgdorferi, remains the most prevalent vector-borne illness in humans. Addressing challenges such as antibiotic resistance, persistent infection, and complex disease dynamics requires identifying novel therapeutic targets. Manganese (Mn²˖) is essential for the...