protein folding

Post-print gold nanometallization tunes the architecture-dependent chemomechanics of 4D protein microstructures

The radical that was never seen: one added hydrogen bond opens up the photoactivation mechanism of a blue-light receptor

A collaboration between researchers in the United States, the United Kingdom and Hungary has mutated the amino acid sequence of the AppA BLUF domain, a blue-light photoreceptor, and succeeded in capturing the proton-coupled electron transfer (PCET) radical intermediates that had never been observed in this protein. By...

The interaction between NC(p7)1-55 and p6 may regulate interactions with nucleic acids during assembly through modulation of Gag folding

Experimental evidence of a direct protein-protein interaction between p6 and the N-terminal region of NC(p7)1-55 is brought and this suggests that p6 could modulate the recognition of the viral genomic RNA (gRNA) by interacting with NC(p7)1-55 during assembly and be a key player in this process.

Supplementary Tables S1-S16 from Spatial Mapping of the Precancer-to-Cancer Transition in Breast and Prostate

Supplementary Table 1. Clinical metadata Supplementary Table 2. 3D stacked serial sections overview Supplementary Table 3. 2D gene discovery sections overview Supplementary Table 4.CODEX panel information Supplementary Table 5. Xenium panel information Supplementary Table 6. HT704B1 distant region path metadata Supplem...

Engineering Caspase-Activated DNase for Nucleosome-Resolution Chromosome Conformation Capture

Chromosome conformation capture methods such as Hi-C and Micro-C have transformed our understanding of genome organisation, revealing architectural features from megabase-scale compartments to kilobase-scale loops. Yet the choice of fragmentation enzyme imposes constraints that shape which features are most readily obs...

Transcriptomic insights into exogenous fatty acid-enhanced halotolerance in Zygosaccharomyces rouxii.

Exogenous C16:1 elicited a focused transcriptional adjustment rather than broad transcriptome-wide reprogramming in salt-stressed Z. rouxii, indicating that peroxisome-linked lipid processes and redox regulation were candidate mechanisms underlying fatty-acid-associated halotolerance and provided targets for improving...

Platelet-erythrocyte complex production is exacerbated by histones and protected by small polyanion histone neutralizing compounds

Platelet-erythrocyte (PE) complexes, composed of single erythrocytes bound to platelets, are a physiological component of the bloodstream and contribute to erythrocyte turnover. Conditions in which PE complex production is increased or their clearance from the circulation is impeded are associated with hypercoagulable...

Platelet-erythrocyte complex production is exacerbated by histones and protected by small polyanion histone neutralizing compounds

Platelet-erythrocyte (PE) complexes, composed of single erythrocytes bound to platelets, are a physiological component of the bloodstream and contribute to erythrocyte turnover. Conditions in which PE complex production is increased or their clearance from the circulation is impeded are associated with hypercoagulable...

Extracellular Vacuole-derived bodies (EVacs) mediate RNA secretion in plants

Extracellular RNAs are found in the plant extracellular space, but how they are exported from cells remains unclear. We found that the plant vacuole is a major source of extracellular RNA and identified a class of large extracellular vacuole-derived bodies, which we termed EVacs, that are key mediators of this transpor...

Pushing the limits of single-cell proteomics to investigate bacterial heterogeneity using bacSCP

Single-cell proteomics (SCP) has emerged as a powerful approach to quantify protein expression variability at cellular resolution, yet most state-of-the-art workflows are tailored to eukaryotic cells with only one study exploring how single bacteria can be analyzed by mass spectrometry. Here, we established bacSCP, a p...

Changes in Activity of Heat Shock Protein-70 Family Genes is Associated With Early Acute Pancreatitis Severity.

OBJECTIVES Acute pancreatitis (AP) is a painful and potentially life-threatening disorder, with no effective therapy available to date. Genome assessment shows promise as a potential method for predicting AP severity. We hypothesized that single nucleotide polymorphisms (SNPs) and expression changes in Heat Shock Prote...

Protein folding as a configurational reconstruction: Bio-epistemic application of the General Epistemic Dispersion Theory to adult human hemoglobin

This manuscript proposes a theoretical application of the General Epistemic Dispersion Theory (GEDT) to the domain of molecular biology, utilizing adult human hemoglobin (HbA) as a model study. The work formalizes protein folding as a configurational reconstruction problem, investigating how informational load is parti...