This study shows that an allosteric activator of AMPK is sufficient to induce RPS24L and autophagy in normoxia in an ULK1-dependent, but mTORC1-independent manner and interacts with RPS24 mRNA and its autophagy-dependent decrease shifts splicing toward the RPS24L variant.
Run-Qi Qian, Jenna Goodbrand, Shannon N. Adams et al.· Biochemistry· 0 citations
LEV and JNJ-42153605 applied briefly after TBI offer a potent polypharmacy approach, at least in the preclinical animal model, to tackle the later tau aggregation and neurodegeneration that follows from TBI neurotrauma.
Laszlo F. Locskai, Shayleen Ghassemi, S. A. Tan et al.· bioRxiv· 0 citations
Testing the ability of common large language models to consider design principles to generate de novo proteins that bind metals and lipophilic small molecules without copying existing sequences highlights the utility of LLMs in making protein design more comprehensible and accessible to users without sophisticated desi...
Nam Hyeong Kim, A. K. Hatstat, Hyunil Jo et al.· bioRxiv· 0 citations
How protein structural differences regulate the performance of protein-polysaccharide nanoparticles is revealed and theoretical and technical insights into the precise design and practical application of delivery systems for LYC and other lipophilic bioactive compounds are provided.
This study provides the first structural basis for pUG- fold RNA recognition by a protein and illustrates how cryo-EM and AI-based methods for protein and RNA structure prediction synergize to inform the mechanism of RNA-mediated regulation of DNMT1.
Jessica J. Song, T. Cech, V. Kasinath· bioRxiv· 0 citations
Among KTRs with T2DM or PTDM, SGLT2i use was associated with a lower combined risk of graft loss, all-cause mortality, or doubling of serum creatinine, without an increased incidence of adverse events.
Secretory vesicle transport from the Golgi to the cell membrane involves kinesin and myosin Va motors on the vesicle surface cooperatively navigating their shared cargo through numerous actin-microtubule (MT) intersections. How the track on which the cargo exits the intersection is selected so that vesicles are deliver...
Brandon M. Bensel, P. Fagnant, Jill E. McFarlane et al.· bioRxiv· 0 citations
A generic numbering scheme for TMEM16 scramblases (GNS-TMEM16), modeled on the Ballesteros & Weinstein system established for class A G protein-coupled receptors, is introduced, bringing to light the advantages of corresponding residues identification in different TMEM16 proteins and show that the mammalian scramblase...
A regulatory role is identified for the conserved, intrinsically disordered C-terminal tails of GroEL, one of the best-studied ATP-dependent chaperones, to reveal how the conformational properties of an intrinsically disordered element can be exploited to optimize the energetic efficiency and functional timing of a lar...
Morgan Powers, Kacie A. Evans, Sun He et al.· bioRxiv· 0 citations
This work describes a label-free colorimetric immunoassay that initially integrates rolling circle amplification (RCA) with Tyndall effect (TE) characteristics of nucleic acid colloids. Using only a laser pointer and a smartphone, it achieves instrument-free protein quantification with 13-fold higher sensitivity than t...
Jia-Rui Li, Wenli Zhu, Karuna D. Chen et al.· Chemical Communications· 0 citations
It is found that reverse folding algorithms are unable to energetically minimize evolutionary conserved frustration at specific residues, even when detrimental to overall structural stability, and it is proposed that these frustration hotspots act as architectural spandrels, inherent physical constraints of the fold th...
Miriam Poley-Gil, Miguel Fernández-Martín, Alin Banka et al.· bioRxiv· 2 citations
A dual pattern of genomic adaptation in Ethiopian zebu cattle is revealed and candidate loci for future validation and climate-resilient livestock breeding are provided.
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
