gene editing

Programmable enzymes for targeted gene insertion.

pgRNA-m6A levels associate with HBV replication and liver function

m6A modification has been shown to play a role in regulating HBV RNA expression. However, it remains unclear whether the m6A levels of HBV pregenomic RNA (pgRNA) are related to HBV replication and liver function. Serum HBV pgRNA-m6A levels were determined by T3 DNA ligase assay. HBV DNA copy numbers and gene expression...

Nanocarrier-Mediated Gene Editing for Improved Tumour Therapeutic Efficacy

This paper reviews considerable recent preclinical literature to systematically summarize the classification, physicochemical properties, and diverse fabrication techniques of mainstream lipid, polymeric, and inorganic nanocarriers and provides reliable systematic theoretical support for the subsequent clinical transla...

Extracellular vesicles in pharmacology: Innovations in drug delivery and therapeutic applications in cancer.

Current therapeutic applications of EVs across breast, lung, colorectal, prostate and pancreatic cancers and glioblastoma are summarized, with emphasis on the EV physicochemical properties and engineered modifications, their cargo and mechanisms underlying their biological effects.

Rational Design of Modular Elastin-like Polypeptide Nanoparticles for Nonviral Gene Delivery

The laboratory's sustained contributions in the ELP field over the past decade are described, culminating in the layer-by-layer elastin-like polypeptide nucleic acid nanoparticle (LENN) platform, which is a modular and biomanufacturable nonviral gene delivery system.

A transdermal CRISPR/Cas9 strategy using biodegradable microneedles to target YB-1 in melanoma

Melanoma's aggressiveness and therapeutic resistance highlight the need for innovative strategies. This study developed a CRISPR/Cas9 ribonucleoprotein (RNP) system targeting the oncogenic YB-1 gene, combined with doxorubicin (DOX), delivered by chondroitin sulfate (CS)-based microneedles (MN) incorporating designed na...

WRRA Life Model IV: Epigenetic Memory and Cell Differentiation

This paper applies the Wonsik Reality-Renderer Architecture (WRRA) to epigenetic memory and cell differentiation. It presents an integrated biological framework connecting DNA sequence, DNA methylation, histone modifications and inheritance, chromatin accessibility, three-dimensional genome organization, mitotic bookma...

WRRA Life Model IV: Epigenetic Memory and Cell Differentiation

This paper applies the Wonsik Reality-Renderer Architecture (WRRA) to epigenetic memory and cell differentiation. It presents an integrated biological framework connecting DNA sequence, DNA methylation, histone modifications and inheritance, chromatin accessibility, three-dimensional genome organization, mitotic bookma...

Recent advances in molecular mechanisms to improve the efficacy of CAR-T cell therapy for viral diseases, cancer, and autoimmune diseases

This review synthesizes recent advances in molecular engineering strategies that enhance CAR-T cell function beyond conventional receptor design and discusses how receptor engineering, genome editing, transcriptional and epigenetic regulation, metabolic reprogramming, synthetic gene circuits, and safety-control platfor...

Mitochondrial genome assembly and annotation of Oryza longistaminata

Oryza longistaminata serves as a critical germplasm resource for rice breeding; however, its mitochondrial genome structure and evolutionary characteristics remain largely unexplored. Here, we report the first complete mitochondrial genome of O. longistaminata assembled using PacBio HiFi long-read sequencing data. Empl...

Mitochondrial genome assembly and annotation of Oryza longistaminata

Oryza longistaminata serves as a critical germplasm resource for rice breeding; however, its mitochondrial genome structure and evolutionary characteristics remain largely unexplored. Here, we report the first complete mitochondrial genome of O. longistaminata assembled using PacBio HiFi long-read sequencing data. Empl...

Appraising the potential of gene editing in Zimbabwe’s crop improvement programmes: a review

Zimbabwe and Sub-Saharan Africa face urgent pressure to feed a rapidly growing population expected to double by 2050, while coping with climate variability. The agriculture sector contributes 15–18% to Zimbabwe’s GDP and over 40% of export earnings. With 70–80% of Zimbabwe relying on rain-fed agriculture, conventional...