gene editing

Next-generation biotechnology for improving abiotic stress tolerance in rice

CRISPR/Cas9-Mediated Editing of Terpene Synthase Gene Reduces Volatile Emission and Attraction of Hypothenemus hampei to Coffea arabica Fruits

The coffee berry borer (CBB), Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae), is one of the most destructive pests of coffee, causing significant losses in yield, bean quality, and overall crop profitability. Current integrated pest management strategies reduce damage but cannot prevent insects f...

Timed Self-Excision of PlWUS-IPT Resolves the Transformation Efficiency-Phenotype Trade- off in Tobacco

Tissue engineering: methods for scaffolds to extracellular matrix and scaffold to cell interaction

Engineering circular RNA expression systems to minimize contaminating linear RNA byproducts

Circular RNAs (circRNAs) are generated by backsplicing of eukaryotic protein-coding transcripts and can regulate microRNAs and RNA binding proteins, or serve as translation templates. Their covalently closed structure confers resistance to exonuclease-mediated degradation, extending their half-life and supporting their...

Evaluation of Alcalase pretreatment for Chlamydomonas reinhardtii CRISPR knock-in

We wanted to use CRISPR-Cas9-mediated homology-directed repair (HDR) in C. reinhardtii to insert genomic landing pads as part of a larger project. In cell-walled algae, delivering Cas9 ribonucleoproteins (RNPs) and donor DNA requires first digesting that wall. The standard approach uses autolysin, a gamete-derived prot...

Beyond Precision: A Multidimensional Framework for Selecting Genetic Medicine Platforms

A multidimensional framework is proposed in which gene therapy platforms are evaluated according to three intrinsic properties—genetic precision, temporal control, and dosage tunability—while delivery, clinical maturity, and disease context act as major translational constraints.

Lipoprotein(a) as an independent cardiovascular risk factor

Emerging evidence links Lp(a) to atrial fi brillation, broadening its clinical relevance and integrating Lp(a) testing into risk assessment and preparing for targeted therapies are critical steps in modern cardiovas-cular prevention.

An inducer-independent, single-plasmid CRISPR-Cas9 system for genome editing in Bacillus species

A one-step CRISPR-based transformation protocol developed in this study enables fast genome editing workflows with minimal hands-on time and demonstrates the versatility of the modified system for this industrially important genus.

The Cucurbita ficifolia Mitochondrial Genome: Repeat-Rich Architecture, Plastid Homology, and Synteny Turnover Across Cucurbitaceae

Background: Cucurbita ficifolia (figleaf gourd) is an agriculturally useful cucurbit germplasm and rootstock, but its mitochondrial genome has not been examined in a comparative family-level framework. We aimed to characterize its mitochondrial reference sequence and evaluate coding composition, repetitive DNA, plastid...

EFFECTIVENESS OF TREATING GENETIC DISEASES BY GENE THERAPY

This article analyzes the effectiveness of gene therapy in treating genetic diseases, its main molecular mechanisms, and modern technologies. Key therapeutic approaches such as gene addition, gene function restoration, gene expression silencing, and targeted genome editing are covered. The potential of modern gene deli...

Aptamer-mediated CRISPR-Cas9 delivery for precision oncology: integrating molecular targeting, intracellular trafficking, and nanocarrier engineering

Cancer therapy remains constrained by insufficient molecular specificity, intratumoral heterogeneity, and the emergence of therapeutic resistance that limit durable clinical responses. CRISPR Cas9 genome editing offers an unprecedented opportunity to directly reprogram oncogenic drivers, tumor suppressor pathways, and...