gene editing

Advances in Wheat (Triticum aestivum L.) Genetics, Genomics and Breeding: Current Status, Tools and Future Perspectives for Sustainable Food Security

Wheat ( Triticum aestivum L.) is one of the most important cereal crops in the world. It supplies nearly 18–20% of global dietary calories and protein, sustaining the food security of over 2.5 billion people. Global food demand is projected to increase by 35–56% by 2050, placing additional pressure on wheat product...

CRISPR/Cas9-mediated generation of a green hypocotyl-marked male-sterile line for hybrid seed production in Solanum lycopersicum

Tomato ( Solanum lycopersicum ) is a self‑pollinating crop that exhibits significant heterosis. While F1 hybrid seeds offer high uniformity and strong stress resistance, the scarcity of male‑sterile materials means that production still relies on manual emasculation and pollination. This labor‑intensive process has bec...

Comprehensive Evaluation and Transcriptome Analysis of Drought Resistance in Castor Bean (Ricinus communis L.)

Castor bean (Ricinus communis L.) is an important industrial oil crop worldwide. In China, western Jilin Province represents a major production region for this species, where spring drought severely constrains its yield and quality.This study aimed to comprehensively evaluate drought resistance in castor bean and explo...

Transforming Plant Viruses into Vectors for Next-Generation Agriculture-A Review

CRISPR-Enabled functional genomics in hPSCs-derived neural models for autism spectrum disorder

This review synthesizes the current state of CRISPR-based functional genomics in human stem cell neural models as a coherent experimental framework for converting ASD genetic associations into mechanistic understanding and therapeutic opportunity.

Functional disruption of vgsc reveals haplosufficiency with implications for insecticide resistance and genetic control in Anopheles gambiae

The insecticide resistance phenotype of vgscKO is investigated and it is demonstrated that knocking out one copy of vgsc produces reduced susceptibility to deltamethrin and DDT at sub-discriminating doses, with implications for vgsc-mediated mechanisms of insecticide resistance.

CRISPR-enhanced assessment of variants of unknown significance nominates oncology therapeutic targets and drug repositioning opportunities

CRISPR-VUS combines dependency-based rare-variant discovery with evidence-guided prioritisation to nominate candidate drivers, therapeutic targets and drug-repositioning hypotheses to nominate candidate drivers, therapeutic targets and drug-repositioning hypotheses.

Novel Therapeutic Frontiers in Duchenne Muscular Dystrophy: Gene Therapy, Exon Skipping, and Stem Cell Approaches.

Through multidisciplinary treatment and continuous scientific progress, individualized precision medicine that integrates therapies targeting secondary pathogenic pathways with dystrophin-restoration techniques can finally convert this devastating illness into a tolerable chronic ailment.

Light-activated CRISPR/dCas9 nanomedicine for programmable control of renal fibrosis.

It is argued that the most defensible therapeutic objective is not permanent genome editing or autonomous organ regeneration, but spatially confined, temporally limited, and reversible regulation of validated fibrotic or protective gene programs using CRISPRa, CRISPRi, or dCas9-based epigenome editors.

Multiplex FAD2/FATB Editing Generates Ultra‐High‐Oleic, Low‐Saturate Soybean With Increased Seed Fatty Acid Content

This study defines effective multiplex target combinations for ultra-high oleic, low-saturated soybean by simultaneously targeting fatty acid desaturase 2 (FAD2) and fatty acyl-ACP thioesterase B (FATB) gene families using CRISPR/Cas9.

CRISPR crops are coming to Europe — why bolder would be better

For farmers and consumers to reap the benefits of gene-edited plants, guidelines for farming gene-edited plants must be implemented with careful thought.

Testing social acceptance of genetic management in threatened bird conservation: the role of information and framing

Content of image described in text. Summary Genetic management of threatened species to reduce extinction risk is sometimes considered controversial, with earlier surveys demonstrating that the general public are more cautious about genetic management than about some other conservation interventions including killing f...