gene editing

Crosstalk among Wnt, Notch, EGF/ErbB, and BMP signaling pathways in intestinal stem cell fate

Background Intestinal stem cell (ISC) proliferation and differentiation are tightly regulated by signaling crosstalk to maintain epithelial homeostasis. Objectives This review synthesizes current evidence on the roles and interactions of Wnt, Notch, EGF/ErbB, and BMP pathways in governing ISC maintenance, self-renewal,...

Novel drug target ADAM8/17 is associated with the occurrence of intrahepatic cholangiocarcinoma via Notch1/Integrinα5 pathway

Novel targeted drugs are currently available for all patients with intrahepatic cholangiocarcinoma (ICC). The goal of this study was to investigate the association between A Disintegrin and Metalloproteinase8/17 (ADAM8/17) and ICC, and to determine whether ADAM8/17 could be a novel target for ICC treatment. The express...

DNAH14 knockout mice as a model of chronic hydrocephalus: cilia and brain-related pathology

Idiopathic normal pressure hydrocephalus (iNPH) is characterized by gait disturbance, cognitive impairment, and urinary incontinence. Emerging evidence suggests that ependymal ciliary dysfunction contributes to the pathophysiology of chronic hydrocephalus, including iNPH. We previously identified DNAH14 as a causative...

Genetic Engineering

### Genetic Engineering — Research Project This research project focuses on the principles, technologies, applications, and emerging developments of genetic engineering and modern molecular biotechnology. The project brings together scholarly work covering genetics, genomics, molecular biology, recombinant DNA technolo...

How Life Is Executed

How Life Is Executed presents an integrated framework for understanding life as a process in which information is executed, material structures are produced, boundaries are maintained, and the capacity for execution is reconstructed across generations. The book applies WRRA (Wonsik Reality-Renderer Architecture)—a fram...

Development of an AAV-Encoded Adenine Base Editor for Duchenne Muscular Dystrophy.

It is suggested that even modest levels of base editing-mediated exon skipping may ameliorate the DMD cardiac phenotype toward a BMD-like state, supporting the translational potential of this dual rAAV base editing approach for DMD cardiomyopathy.

Generation of Genetically Engineered Embryos Using Gene Editing and Somatic Cell Nuclear Transfer for Production of Sheep Models of Human Disease.

Large animal models are valuable tools for investigating human disease. Sheep, pigs, and goats often better recapitulate the anatomy and physiology of human organs and the complexity of human disease, thereby enhancing their clinical relevance compared to rodents. CRISPR-Cas9 and somatic cell nuclear transfer (SCNT) en...

Russel88/CRISPRCasTyper: v1.9.0

Python-native pipeline: CRISPR detection, HMM searches, and ORF prediction now run in-process (diced, pyhmmer, pyrodigal-gv); database/models ship with the wheel/sdist. Native repeat search backend: bundled edit-distance search (myers-batch + edlib) as a drop-in for BLAST+ near cas operons. --repeat_search_backend defa...

Genetic and molecular basis of gametophytic self-incompatibility in apple (Malus × domestica Borkh.): S-allele diversity, pollinizer selection, and prospects for cultivar improvement

FigureLab: a browser-based tool for assembling publication-quality scientific figures

FigureLab is a single-file, browser-based application for assembling publication-quality scientific figures from image data (microscopy, western blots, histology, gels, and clinical or specimen photographs). It runs entirely client-side with no installation, server, or internet connection required, so no image data eve...

The longevity gene APOE2 enhances pericyte function and reduces lipid droplets.

Pericytes are critical for maintaining blood-brain barrier (BBB) integrity and have emerged as key contributors to Alzheimer's disease (AD) pathogenesis. Although the apolipoprotein E2 (APOE2) allele is associated with reduced AD risk and increased longevity, its impact on pericyte function is unclear. We measured peri...

Overcoming delivery barriers of large all-in-one CRISPR/Cas9 plasmids using lysine-based lipid nanoparticles

Introduction Lipid nanoparticles (LNPs) are widely used as drug delivery systems (DDS) for the intracellular delivery of gene-editing tools such as the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system. Previously, a lysine-headgroup cationic lipid (K3C16) was...