gene editing

Nucleofection-Based CRISPR/Cas Delivery in Human T Cells for Immunotherapy Applications.

Electroporation-based delivery of CRISPR/Cas systems has emerged as a powerful and versatile approach for gene editing in primary human T cells, enabling efficient, transient, and nonviral modification while minimizing genomic integration risks. This chapter focuses on the principles and practical implementation of ele...

Quantitative Analysis of CRISPR Encoding Plasmid DNA–Nanocarrier Interactions by Gel Electrophoresis and Densitometry

Nanoparticle-based carriers are key systems for the transport and delivery of nucleic acids in gene therapy, RNA therapeutics, and genome editing applications. The development of efficient delivery systems for CRISPR–Cas technologies requires reliable methods to evaluate nanocarrier–nucleic acid interactions, binding e...

CRISPR/Cas Systems: Biological Basis and Genome Editing Applications.

Clustered regularly interspaced short palindromic repeats (CRISPR) and associated (Cas) systems have revolutionized the field of genome engineering by providing versatile, efficient, and programmable tools for precise genetic manipulation. Originally identified as an adaptive immune mechanism in prokaryotes, CRISPR/Cas...

Beyond conventional immunosuppression: tailoring perioperative management protocols for clinical xenotransplantation.

Clinical xenotransplantation offers a conceptually unlimited supply of donor organs, owing to transformative advances in gene-editing technologies. However, the pharmacological and physiological distinctions between allo- and xenotransplantation pose a major challenge. Unlike allografts, xenografts simultaneously trigg...

Systematic Profiling of ssODN Determinants Establishes Optimized LbCas12a-Mediated HDR for Precise Genome Editing in Flammulina filiformis

The results not only establish a versatile precision-editing platform for F. filiformis through optimized ssODN design, but also offer tentative and transferable insights into the HDR-mediated genome editing of other heterokaryotic basidiomycetes facing similar genetic operation challenges.

Overview of Delivery Methods for Gene Editing.

The clinical success of CRISPR-based interventions depends primarily on the efficient delivery of editing components into target cells. While base and prime editing have refined genomic precision, achieving therapeutic efficacy requires specialized vehicles that can navigate systemic circulation, escape endosomes, and...

An Overview of Programmable Epigenetic Editing Based on CRISPR Tools for Gene Expression Regulation.

Epigenetic regulation provides a dynamic and reversible layer of gene control that functions independently of changes in DNA sequence, primarily mediated by DNA methylation, histone modifications, and higher-order chromatin organization. Aberrant epigenetic states contribute to a wide range of human diseases. However,...

Plant DNA Damage Response and Repair Pathways: from Molecular Mechanisms to Crop Improvement

This review synthesises the molecular basis of plant DNA damage responses and major repair pathways, with emphasis on their coordination and relevance to crop improvement.

Oligo-Modified Poly(β-Amino Ester)-Mediated mRNA Delivery: A Protocol for Gene Editing Applications

Oligopeptide-modified poly(β-amino ester)s (OM-pBAEs) are polymer-based delivery systems that enable the encapsulation and intracellular transport of nucleic acids, including messenger RNA (mRNA) and mixtures of mRNA with DNA. OM-pBAEs form cationic nanoparticles (NPs) through electrostatic interactions with nucleic ac...

Editorial: Stress tolerance in sorghum: molecular mechanisms, gene discovery, and quality dynamics

Climate change-driven abiotic stressesdrought, salinity, and waterloggingare the primary yield-limiting barriers for sorghum cultivation worldwide, and six papers in this collection dissect the morphological, physiological, and genetic regulatory networks governing sorghum stress tolerance. Drought is the most pervasiv...

Converging GWAS and Genome Editing Decrypts MsCBF8‐ MsGolS2 Regulatory Module for Drought Tolerance Without Yield Penalty in Medicago sativa

ABSTRACT Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome‐wide association study (GWAS) was conducted in alfalfa, which identified a CBF/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR‐Cas9 system,...

Guide reselection expands canonical PAM candidates in an HGD editing resource for alkaptonuria