gene editing

Spatiotemporal Control of Genetic and Epigenetic Editing through Covalent Tethering of CRISPR Nanoparticles to Zwitterionic Microgels

A defined biorthogonal conjugation system that tethers non-viral nanoparticles to biomaterial platforms for sustained and localized CRISPR gene editing is established.

Advance in Life Sciences: Present and Future – A Research Perspective

ABSTRACT Life sciences represent one of the most dynamic and transformative scientific domains in the modern world. Rapid technological progress, interdisciplinary research integration, and molecular-level understanding of biological systems have revolutionized healthcare, agriculture, environmental protection, and bio...

Network Organization Of Genetic Susceptibility to Disease

The file dohai_thesis_core_v1.zip contains the analysis scripts and associated input files used for the doctoral thesis titled “Network Organization of Genetic Susceptibility to Disease”. The scripts are organized according to the analytical workflow described in the Methods chapter. The workflow includes harmonization...

Network Organization Of Genetic Susceptibility to Disease

The file dohai_thesis_core_v1.zip contains the analysis scripts and associated input files used for the doctoral thesis titled “Network Organization of Genetic Susceptibility to Disease”. The scripts are organized according to the analytical workflow described in the Methods chapter. The workflow includes harmonization...

Supporting data for “Transfer RNA Abundance and Modification Shape Codon-Dependent Translational Adaptation in Hepatocellular Carcinoma”

This dataset was generated as part of a research project investigating how transfer RNA (tRNA) abundance and tRNA modification regulate codon-dependent translation in hepatocellular carcinoma (HCC). The data cover two complementary aspects of tRNA-mediated translational regulation.The first project examines the relatio...

Abstract A020: A SMARCA1/SMARCA5 double-knockout-repressed ISWI chromatin-state program marks fusion-enriched pediatric sarcoma models and an ISWI-complex dependency signal in rhabdomyosarcoma

The relationship between DKOdown and multiple chromatin-remodeling family programs supports a model in which coordinated chromatin-remodeler activity may contribute to lineage dysregulation, adaptive plasticity, and ISWI-associated vulnerability in rhabdomyosarcoma and related fusion-enriched sarcoma models.

Supporting data for "Uncovering cellular response to ionizable lipid-induced endolysosomal damage with multimodal imaging"

Lipid nanoparticles (LNPs) are the leading non-viral platform for nucleic acid delivery, validated clinically by their use in mRNA vaccines and the siRNA drug patisiran. Nevertheless, their wider use in protein-replacement and gene-editing therapeutics need repeated doses is limited by inflammatory side effects caused...

Production of Lentiviral Vectors Encoding the CRISPR-Cas13d System for RNA Targeting.

A detailed protocol for the production of high-titer, third-generation LVs with a dual-expression cassette for Cas13d and a customizable guide RNA is described, which can be used for stable transduction and efficient RNA knockdown in a broad range of mammalian cell types.

Adeno-Associated Viral Vector (AAV)-Mediated In Vivo CRISPR-Cas9 Delivery.

CRISPR-based gene editing is a growing therapeutic strategy for modifying or silencing disease-causing genes. This chapter focuses on the in vivo delivery of CRISPR systems using recombinant adeno-associated viral (rAAV) vectors. Specifically, we detail a methodology using a rAAV vector carrying Staphylococcus aureus C...

Establishment of a high-efficiency hairy root transformation system for Forsythia suspensa and enhancement of transformation efficiency by developmental regulators

Forsythia suspensa (Thunb.) Vahl is an economically important woody medicinal plant, yet the lack of efficient genetic transformation protocols hinders genomic and molecular breeding studies. Conventional Agrobacterium tumefaciens -mediated transformation faces low efficiency and long culture cycles, while Agrobacteriu...

Emerging insights and breakthroughs in genetic advancement and phytopathology of Sclerotinia stem rot in rapeseed-mustard

Production and Storage of High-Capacity Adenoviral Vectors for the Delivery of Advanced CRISPR Systems.

Genome editing based on engineered CRISPR systems is advancing rapidly, with the field increasingly moving toward approaches that avoid the induction of mutagenic double-stranded DNA breaks (e.g., RNA-programmable base editing, prime editing, and donor DNA transposition). These nuclease-free strategies often rely on la...