gene editing

Fine-Tuning Electroporation and AAV Delivery Parameters Balances Embryo Developmental Competence and Genome Editing Efficiency

Genetic and Molecular Basis of Processing Traits and Genetic Improvement Progress in Major Fruit Crops

Fruit processing plays a critical role in reducing postharvest losses, enhancing value addition, and improving the utilization efficiency of horticultural produce. However, most fruit breeding programs have historically prioritized fresh‐market traits such as yield, appearance, shelf life, and disease resistance, whe...

Phenotype-genotype association for the identification of resistance to stem borers (Diatraea spp., Lepidoptera: Crambidae) in sugarcane

Sugarcane ( Saccharum spp.) is a major crop for sugar production, but its productivity is affected by stem borers ( Diatraea spp.). These insects cause significant losses by damaging the stem and disrupting the transport of photosynthates and nutrients. Although biological control has been effective in Colombia,...

ZmWGSL2 Is a Candidate Chloroplast-Localized PPR Gene Associated with Chloroplast Development and Leaf-Colour Formation in Maize

Efficient chloroplast development underpins photosynthetic efficiency, biomass accumulation, and ultimately crop productivity. In this study, we characterized wgsl2-0, a stable white-green striped leaf mutant isolated from an ethyl methanesulfonate-mutagenized RP125 population of maize (Zea mays L.). The mutant maintai...

Gene editing technologies in poultry: advances, applications, and future prospects

SUMMARYThe chicken is an important model organism for research and a major global source of animal protein (eggs and meat). Recently developed genome-editing technologies have allowed the production of genetically engineered animals for agriculture and food production. Genome editing in chicken is a promising approach...

CRISPR/Cas9 Gene Editing of OsSD1 and OsDEP1 Improves Plant Architecture and Yield in Indigenous Black Rice Cultivar Chakhao Amubi

Rice ( Oryza sativa L.) sustains over half of the global population. The black rice landraces of North-East India are agronomically limited by tall stature, lodging, and low yield. We employed CRISPR/Cas9 -mediated multiplex genome editing to improve the elite aromatic black rice cultivar Chakhao Amubi by targeting thr...

Chromosome Editing Using the Lambda Red Recombinase System in Chlamydia trachomatis and Chlamydia muridarum

Genome edited sheep generated by zygote electroporation of prime editor ribonucleoproteins

Prime editing (PE) enables precise genome modifications without double-strand breaks, yet its application in large mammals has been hindered by low efficiency and the technical bottlenecks of zygote microinjection. This study aimed to establish a scalable PE platform in sheep via the direct electroporation of a PE3 R...

Genome editing frontiers for developing abiotic stress tolerant and climate resilient crops

Gene editing applications, opportunities and challenges for crop improvement in Africa a review

Gene editing, particularly CRISPR/Cas-based new genomic techniques (NGTs), offers significant opportunities for accelerated crop improvement in developing African countries, ensuring food security, livelihoods, climate resilience, and economic growth. This review critically evaluates the scientific basis, applications,...

Hypertrophic Wasp Stem Cell Bio-Hybrid Muscle Actuator Powered and Cryogenically Cooled by Ethanol Solution

A novel theoretical concept in bio-hybrid robotics and tissue engineering: a macroscopic biological muscle actuator engineered from the stem cells of the Oriental Hornet (Vespa orientalis). The system utilizes CRISPR-Cas9 genome editing to multiply the Alcohol Dehydrogenase (NADP+) gene for absolute alcohol immunity, c...

Language-Model-Based Detection of Genetic Editing in Bacteria

Recent advances in genome editing allow easy genetic manipulation of bacteria, providing them with new traits, some of which could be hazardous, e.g. enhanced virulence or extended resistance to antibiotics. The ability to detect artificially modified bacteria is crucial for identifying potential bio-threats. However,...