Files comprising raw values, figures and supplementary materials for the paper: "Development of a novel photocurable collagen-methacrylated hyaluronic acid-based bioink mimicking synovial extracellular matrix"

Abstract

The research surrounds the development and characterisation of a novel 3D-printable synovial tissue analogue (CHA-M), comprised of type I collagen, methacrylated hyaluronic acid and 4-arm PEG SCM. Given the increasing prevalence of bioprinting for 3D modelling and the use of extrusion-based bioprinting for synovial modelling, CHA-M was not only formulated as a tissue analogue, but adapted as a bioink for this technology. This research explores the development of CHA-M, with the data presented detailing the following characterisations: physiochemical and chemical, photo-cross linking kinetics, microstructure, nutrient diffusion and degradation testing. Cytocompatbility and initial printing trials are also supported by the data presented here. Figure 1 - Initial printing matrix for 3D printing trails using bioink.Figure 2 - Figure displaying sample rheological data for different bioink formulations and sample viscosity at low/high shear rates.Figure 3 - Figure displaying photo-crosslinking kinetics of different bioink formulations and sample viscosity at different temperatures.Figure 4 - Figure showcasing bioink formulation and crosslinking strategy, FTIR fingerprint region of hyaluronic acid, FTIR spectra of different bioink formulations and components.Figure 5 - SEM imaging data of pre-hydrogel and hydrogel formulations pre/post photo-crosslinking.Figure 6 - Figure showcasing nutrient diffusion and degradation properties of the final hydrogel formulation for both low and high molecular weight hyaluronic acid.Figure 7 - Cytocompatibility testing of hydrogel with THP-1 cells; cell viability data and images (Live/Dead, morphology)Figure 8 - Cytocompatibility testing of hydrogel with FLS cells; cell viability data and images (Live/Dead, morphology)Figure 9 - Bioink printing matrix, printing trial results and printing resolution images and data. Live/Dead images of FLS cells 24hrs post-printing.Table 1 - Hydrogel formulations and gelation images.Table 2 - FTIR data of Amide A, I, II, III for hydrogel components, pre-hydrogel and final hydrogel pre/post-photo crosslinking.Supplementary Figure 1 - Figure displaying sample rheological data for different bioink formulations at 20 degrees.Supplementary Figure 2 - Sample viscosity of the final hydrogel formulation at low and high shear.Supplementary Figure 3 - Cytocompatibility testing of hydrogel with Neo-NHDF cells; cell viability data and images (Live/Dead).Supplementary Table 1 - Average viscoelastic and photo-crosslinking values over time, from microholder CHA-M samples.Supplementary Table 2 - Changes in viscoelastic and photo-crosslinking values over time (at 2 and 8 minutes post-UV initiation), for microholder CHA-M samples.