Harnessing Affinity Tags for Recombinant Protein Purification: Concepts, Methods, and Troubleshooting.

The development of BioID: A tool for proximity proteomics in living cells.

Mapping and identification of proteins and protein complexes in cells is of fundamental importance when studying protein functions and biological mechanisms. Conventional methods, such as affinity purification coupled with mass spectrometry (AP-MS) and yeast two-hybrid screening, have commonly been employed to study pr...

Expression and purification of recombinant Protein L variants in Escherichia coli.

Protein L is a κ light-chain-binding protein, which is widely used as an affinity ligand for antibody purification and characterization. However, manufacturable recombinant Protein L ligands remain insufficiently studied, particularly regarding how domain architecture affects production, processability, and product qua...

A generalizable method for the purification and biophysical characterization of bacterial membrane receptors

Membrane‐embedded bacterial receptors are challenging to express and purify in soluble form, yet their isolated domains are essential tools for structural and ligand‐discovery studies. Pseudomonas aeruginosa relies on the TonB‐dependent heme receptor HasR for iron acquisition, a process central to its pathogenicity. He...

Engineering a modular p2GUS expression system for enhanced Ni-binding protein production in Escherichia coli

Overcoming the Limitations of Protein A: Evolution of Bacterial Protein-Based and Synthetic Affinity Ligands for High-Performance IgG Purification

The key finding is that the reliable and time-tested resins like those based on protein A continue to dominate the market, although future trends also point toward smaller, more stable, and cost-effective synthetic ligands for specific applications.

[Screening of highly active recombinant bovine enterokinase mutants mediated by green fluorescent protein and its soluble expression in Escherichia coli].

The soluble expression of EKL in E. coli is achieved and a highly active mutant is obtained, and a visual tool for the rapid detection of EKL activity is provided.