[Screening of highly active recombinant bovine enterokinase mutants mediated by green fluorescent protein and its soluble expression in Escherichia coli].
Sep 2026· Sheng wu gong cheng xue bao = Chinese journal of biotechnology· Vol 42 9, pp.
4038-4051
· 0 citations
Medicine
TL;DR
The soluble expression of EKL in E. coli is achieved and a highly active mutant is obtained, and a visual tool for the rapid detection of EKL activity is provided.
It is demonstrated that PDI(a)-mediated cis-fusion enables high-yield production of structurally native-like and functionally competent HSA in the bacterial cytosol and provides a practical platform for producing complex disulfide-rich proteins in E. coli.
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