Author

T. Kumar

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Open access Sep 2026

Enhancing the Scalable Manufacturing of Human Mesenchymal Stromal Cells in Dynamic Culture Using Bioactive Heparin/Collagen Layer‐by‐Layer Coated Microcarriers

It is demonstrated that heparin/collagen Layer‐by‐Layer (LbL) coatings enhance hMSC functional performance and scalability of microcarrier‐based culture, especially at lower initial cell densities, representing a promising approach for scalable cell manufacturing with the potential for GMP adaptation.

Justin Putman, Varsha Karunakaran, Roaa Hadi et al. · Biotechnology Journal · 0 citations

2026

Harnessing Affinity Tags for Recombinant Protein Purification: Concepts, Methods, and Troubleshooting.

Recent advancements of design and functions of different affinity tags namely, polyhistidine (His-tag), glutathione S-transferase (GST), small ubiquitin-like modifier (SUMO) tag, maltose-binding protein (MBP), calmodulin-binding protein (CBP), intein-chitin-binding domain tag, N-utilization substance A tag, Halo Tag®,...

O. Edirisinghe, Kaynat Shahzad, Lawrencia Kumi et al. · Methods in molecular biology · 0 citations