2-Pyrone-4,6-dicarboxylic acid (PDC) is a promising pseudo-aromatic bio-based monomer for the production of biodegradable polyesters. Here, we combined metabolic engineering with fermentation process optimization to establish efficient de novo PDC biosynthesis in Corynebacterium glutamicum. To enhance the availability of protocatechuic acid (PCA), the direct precursor of PDC, endogenous PCA degradation was blocked, and precursor supply through the shikimate pathway was strengthened. Subsequently, the ligABC cluster from Sphingomonas paucimobilis SYK-6 was heterologously introduced to enable PDC formation from glucose. Medium screening identified a corn steep liquor-containing formulation that increased the PDC titer by 1.4-fold in shake-flask cultures. Further optimization of corn steep liquor concentration, dissolved oxygen, and glucose feeding rate in a 5-L bioreactor increased the PDC titer to 71.1 g/L at 90 h, with an average productivity of 0.8 g/L/h, the highest productivity for C. glutamicum-based PDC production reported to date.
Halomonas is demonstrated as a competitive non-sterilized platform to produce poly(3-hydroxybutyrate) (PHB) presenting a potential biomanufacturing scenario for the transformation of severely damaged agricultural debris.
Lei-Zhen Zhao, Lin Chen, Gao-Jie Sun et al.· ACS Sustainable Chemistry &a...· 0 citations
A single-vessel two-step chemobiocatalytic strategy was developed for conversion of d-fructose, bread crumbs, and marine biomass (purple laver) into value-added furanic chemicals. A ternary deep eutectic solvent (DES), Be/Gly/MaA, efficiently catalyzed 5-hydroxymethylfurfural (5-HMF) production from d-fructose, bread...
1,3-Propanediol is a versatile C3 diol serving as a key building block used in various industries. Klebsiella pneumoniae represents an attractive microbial chassis for 1,3-PDO production due to its endogenous 1,3-PDO and Vitamin B₁₂ biosynthetic pathways. However, low carbon flux and by-product formation remain bottlen...
Shao-Qi Sun, Ai Chen, Wen-Qi Wang et al.· Journal of Biotechnology· 0 citations
cis-1,4-Polyisoprene, the primary component of natural rubber, is a critical industrial polymer traditionally sourced from Hevea brasiliensis or petroleum. Microbial production offers a sustainable alternative, but achieving efficient synthesis and high molecular weight remains challenging. Here, we report the de nov...
Xiaoli Zhao, Xinxi Li, Yuzheng Ma et al.· ACS Sustainable Chemistry &a...· 0 citations
Efficient biosynthesis of 1,3-PDO, a key bio-based chemical, depends on precise regulation of the host metabolic network. In this study, a heterologous CRISPR-Cas12a genome editing system was established and systematically optimized in Klebsiella pneumoniae, enabling efficient and stable genome editing (75-100% efficie...
Li Wang, Ming-Yang Zhao, Yuan-Ming Ye et al.· Bioresource Technology· 0 citations
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