Evolutionary stabilisation of stressful metabolism via integrated biocomputing and essential-gene metabolic locking circuits

Species-specific metabolic networks shape evolutionary routes to functional rescue

Metabolic networks are highly interconnected. Still, it remains unclear whether organism-and environment-specific factors shape their capacity to evolve in response to metabolic stress or whether it follows general principles. Here, we studied metabolic evolvability in Bacillus subtilis using auxotrophic mutants lackin...

Multidimensional metabolic engineering strategies for efficient production of branched-chain amino acids and their derivatives in Escherichia coli and Corynebacterium glutamicum.

This review presents a comprehensive analysis of recent advances in microbial BCAAs production through a Mechanism-Module-Process framework, providing a holistic roadmap for constructing superior BCAAs cell factories and extending their metabolic potential toward derivative biosynthesis.

Progressive strain development of industrially relevant microbes via genome editing and systems biology

Metabolic adaptation to TCA cycle deficiency in a strictly aerobic bacterium

The tricarboxylic acid (TCA) cycle is central to cellular metabolism, yet its disruption offers an attractive opportunity to redirect carbon toward biotechnological products. A key challenge is that severe TCA cycle interruption often results in auxotrophic phenotypes, limiting their practical application. Here, we use...

Interaction-driven chorismate metabolic reprogramming underlies Bacillus–Pediococcus synergistic thermogenesis in high-temperature Daqu

High-temperature Daqu (HTD), the essential solid-state fermentation starter for premium Moutai-flavor Baijiu, reaches a peak temperature of 60–65 °C driven by microbial metabolic heat; yet the detailed metabolic mechanism underlying the Bacillus–Pediococcus thermogenic synergy remains largely unresolved. This study int...

Meeting gene expression demand during evolution of antibiotic resistance.

Regulated gene expression in bacteria accommodates demands imposed by different environments. In addition to ensuring the production of required metabolic and structural components, gene regulation protects bacteria against the adverse effects of misexpression. Upon encountering a novel environment, such as sustained e...