Oct 2026· BIOspektrum· Vol 32, pp. 629 - 631· 0 citations· 4 references
TL;DR
Super-resolution microscopy is applied on Ca V 1.3 channels in stem-cell derived atrial myocytes to study cluster architectures and dynamics, finding that these channels are mobile inside membrane domains, being loosely clustered in contrast to previous models of dense channel packing.
Abstract
Ca
2+
influx into cells occurs through Ca
2+
-conducting channels, activating special functions in myocytes and neurons. L-type Ca
2+
channels are confined in sub-micrometer-sized membrane regions. How they cluster there is poorly understood, while this is relevant for functional regulation. We applied super-resolution microscopy on Ca
V
1.3 channels in stem-cell derived atrial myocytes to study cluster architectures and dynamics. Surprisingly Ca
V
1.3 channels are mobile inside membrane domains, being loosely clustered in contrast to previous models of dense channel packing.
CARMEN is introduced, a polycistronic fluorescence-based biosensor system that enables simultaneous, high-resolution monitoring of Ca 2+ signals in distinct subcellular compartments within the same living cell, supporting future high-content imaging and drug-screening applications.
Ca2+ is a secondary messenger that controls a wide range of processes. In vascular endothelial cells, cytosolic Ca2+ is known to be affected by shear stress. Therefore, imaging Ca2+ activity at cellular and subcellular levels in endothelial cells helps to study effect that shear stress has on cellular functions. In thi...
Myelin membranes are tightly connected to the axon, forming an electrical route for rapid saltatory conduction of action potentials from node to node. Beneath the myelin, adjacent to the node, lie voltage-gated potassium (K+) channels, whose fundamental role in conduction remains poorly understood. Constrained by biolo...
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Neurovascular coupling (NVC), initiated by the brain’s dense capillary network, matches blood flow to neuronal activity, yet key underlying mechanisms remain unresolved. We found that ORAI1 channels and their regulator STIM1, the main drivers of store-operated Ca2+ entry, are essential for communicating neuronal metabo...
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BACKGROUND
The myogenic response is the key autoregulatory mechanism that sets cerebral blood flow, and its mechanistic foundation is intimately tied to depolarization and the voltage gating of L-type Ca2+ channels (CaV1.2). Although critical, this study argues for an additional mechanism. We hypothesize that increased...
G. Mironova, Miguel A. S. Martín-Aragón Baudel, C. Lambride et al.· HYPERTENSION· 0 citations
Calcium- and calmodulin-dependent protein kinase II (CaMKII) is highly enriched in dendritic spines at concentrations comparable to those of cytoskeletal proteins and plays a central role in synaptic plasticity. During long-term potentiation, CaMKIIα further accumulates in spines. However, the mechanisms governing its...
Taisei Suzuki, T. Sumikama, Keisuke Matsushima et al.· Science Advances· 0 citations
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