The m⁶A reader YTHDF2 cooperates with FMRP to regulate distal mRNA transport and axon outgrowth in developing neurons

Abstract

Table S1. The list of Mettl14-dependent m6A peaks identified by m6A -SAC-seq. The table includes the chromosome, chromosomal position, original genome sequence, mutated sequence, mutation ratio among transcripts, gene symbol, and the genomic location of m6A peaks identified using m6A-SAC-seq by comparing P0 WT and M14 cKO mouse forebrain. Table S2. The list of mRNAs with significantly altered abundances between the corpus callosum of WT and M14 cKO mice. The table includes the Ensembl transcript ID, gene symbol, baseMean count, log2 fold-change ratio, and adjusted p-value. Results were filtered according to the standard described in the Methods section (provided as a separate Excel spreadsheet). Table S3. The results of the RNA stability assay in Mettl14 and Ythdf2 cKO primary cortical neurons. The table includes the gene symbol, the log2 fold-change ratio in the 0-hour sample, the log2 fold-change ratio in the 5-hour sample, the difference between the 0-hour and 5-hour samples, and the presence of m6A from the m6A-SAC-seq results. The first sheet shows the stability assay results for the M14 cKO condition compared to the control. The second sheet shows the stability assay results for the DF2 cKO condition compared to the control. The third sheet compares the M14 cKO and DF2 cKO stability assay results. Results were filtered according to the standard described in the Methods section (provided as a separate Excel spreadsheet). Table S4. The list of protein interactors of YTHDF2 identified by Co-IP LC-MS/MS experiments. The table includes the gene symbol, the normalized PSM values for WT and M14 cKO with IgG pull-down samples, and the log2 fold-change of PSM values in WT compared to M14 cKO (provided as a separate Excel spreadsheet). Table S5. Information on primers used for qPCR analysis. The table includes the gene symbol, forward and reverse primer sequences, NCBI gene ID, and NCBI transcript ID.