AI Networking Cookbook: Practical recipes for AI-assisted network automation and development
TL;DR
A protocol-detailed herein-combining the immunoprecipitation of a fibrillarin-Yellow Fluorescent Protein (FIB2-YFP) fusion protein in Arabidopsis thaliana with high-sensitivity liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis is developed.
The U1 small nuclear ribonucleoprotein (U1 snRNP) is an essential spliceosome component that mediates pre-mRNA splicing. Although the molecular function of Luc7 proteins is broadly conserved in eukaryotes, whether this core splicing factor acquires developmentally specialized functions in filamentous fungi remains uncl...
The Tpt1 homologs of kinetoplastid protozoa are bifunctional enzymes composed of an N-terminal DEDDh-type nuclease domain linked to a C-terminal 2'-phosphotransferase domain, which can remove a 2'-terminal phosphate via transfer to NAD+, albeit much less efficiently than removal of an internal 2'-phosphate.
Biochemical, RNA and structural analyses connect AGO1 to snoRNP machinery and identify AGO1 as a nucleolar regulator that associates with Fibrillarin-containing snoRNP machinery and links small-RNA biology to pre-rRNA homeostasis and cell growth.
Mitochondrial pre-mRNA editing in kinetoplastids, a clade that includes human-infective parasitic protists such as Trypanosoma and Leishmania, is required to produce translatable, functional gene products from otherwise nonfunctional mitochondrial precursor transcripts of essential respiratory chain complex subunits. R...
RNA editing in mitochondria is vital for many eukaryotes. In plants, mitochondrial C-to-U deamination editing is catalyzed by tens to hundreds of PPR-DYW proteins, each typically dedicated to a specific site. Where retained, PPR-DYW family expanded independently in all eukaryotic groups examined here—except in marine m...
Poly(A) Binding Protein Nuclear 1 (PABPN1) is a ubiquitously expressed nuclear protein that is primarily known for its stimulatory role in poly(A) tail synthesis. PABPN1 is also involved in several other aspects of RNA processing, including splicing, alternative polyadenylation and nuclear RNA surveillance, but these f...
We use cookies to run the site and, with your consent, for analytics and to show ads. See our Cookie Policy.