Jul 2026· Genetics and Molecular Research· 0 citations· 20 references
TL;DR
It may be suggested that the lotus petals methanolic extract can bring hepatorenal and lipid metabolic balance to normal condition and the dose of 750 mg/kg exhibited the maximum response with respect to the other doses of lotus petals methanolic extract tested.
Abstract
Background: Carbon tetrachloride (CCl₄) is a common experimental toxicant which causes a high degree of serum biochemical changes associated with liver, kidney and lipid metabolic dysfunctions. The Nelumbo nucifera has been reported to have significant pharmacological properties such as antioxidant and hepatoprotective property. Thus, the present study aimed at evaluating the biochemical effects of lotus petals methanolic extract of N. nucifera against CCl₄ induced toxicity in albino Wistar rats. Methodology: The lotus petals methanolic extract was obtained from the fresh N. nucifera petals. Albino Wistar rats were further subdivided into four groups: control group, CCl₄ only group, lotus petals methanolic extract at 250, 500 and 750 mg/kg b.w. and silymarin standard group. The protective effect of the lotus petals methanolic extract was investigated by serum biochemical markers such as AST, ALT, ALP, LDH, bilirubin, urea, creatinine, triglyceride and cholesterol. Results: Serum hepatic enzyme markers, bilirubin, renal function markers and lipid profile markers were significantly raised after administration of CCl₄, suggesting biochemical disruption and systemic toxicity. Treatment with lotus petals methanolic extract improved all the serum biochemical parameters in a dose dependent manner. The lotus petals methanolic extract treated group at the highest dose (750 mg/kg) was the most protective against the CCl₄ induced alteration of the AST, ALT, ALP, LDH, bilirubin, urea, creatinine, triglyceride and cholesterol. Conclusion: Lotus petals methanolic extract showed significant protective effect in terms of serum biochemical parameters against CCl₄-induced toxicity. From the result obtained it may be suggested that the lotus petals methanolic extract can bring hepatorenal and lipid metabolic balance to normal condition and the dose of 750 mg/kg exhibited the maximum response with respect to the other doses of lotus petals methanolic extract tested.
Pesticide exposure is a growing health concern globally because of its toxic effects on vital organs. Present research was conducted to investigate the effects of bromoxynil on the liver and kidney, along with the protective potential of Nigella sativa extract. Experimental animals were divided into four groups: control(Co), Nigella sativa extract alone(NS), bromoxynil-treated (BO), and combined treatment(BO + NS). Histological, micrometric and blood chemical parameters were measured following 10,15- and 25-days experimental period. Biochemical analysis revealed that bromoxynil exposure significantly increased serum liver enzymes (ALT, AST, ALP) and renal function markers (urea, creatinine), over different experimental periods indicating hepatotoxicity and nephrotoxicity. Hematological alterations including reduced red blood cells and hemoglobin with increased white blood cells reflected systemic stress. Histological examinations confirmed these findings, where bromoxynil caused severe hepatic alterations such as vacuolated hepatocytes, pyknotic nuclei and disrupted sinusoidal architecture while kidney sections showed shrunken glomeruli, widened periglomerular spaces and degenerated tubular cells. In contrast, animals treated with Nigella sativa displayed marked improvement. The extract stabilized liver and kidney functions restored hematological balance and preserved normal tissue structures. Overall, our findings highlight the dual threat of bromoxynil toxicity and the therapeutic potential of Nigella sativa in protecting vital organs against pesticide induced damage.
A. Batool, J. Ikram, Muhammad Fayyaz Ur Rehman et al.· Journal of Basic and Applied...· 0 citations
S. aethiopicum leaf extract protected against potassium bromate-induced hepatic dysfunction and inflammatory disturbances, supporting its potential as a natural hepatoprotective and immunomodulatory agent.
Okon Victoria Edem, Idam Benedict Umagu, I. Nnah· IAA Journal of Biological Sc...· 0 citations
This study provides the first demonstration that asiatic acid exerts robust systemic antioxidative, hepatoprotective, and neuroprotective effects against CPF-induced toxicity, highlighting AA’s therapeutic potential for mitigating organophosphate pesticide poisoning.
Özge Darakcı Saltık, H. Balcı, Figen Güzelgül et al.· Yüksek İhtisas Üniversitesi...· 0 citations
INTRODUCTION/OBJECTIVE
Digera muricata (DM) contains phenolic compounds and quercetin and β-caryophyllene, which exhibit anticancer, anti-inflammatory, and antioxidant activities. The present study was conducted with the objective of examining the hepatoprotective role of DM ethanolic extract in a hepatotoxic model of Sprague-Dawley rats.
METHODS
Sprague-Dawley (SD) male rats were divided into the following groups: Normal Control (NC); hepatotoxic disease control (DC); hepatotoxic group treated with a low dose (LD) of DM (LDDM-INH+RIF); hepatotoxic group treated with a high dose (HD) of DM (HDDMINH+RIF), and reference control (RC) standard drug silymarin for 21 days. The hepatoprotective action was explored by measuring various liver function enzymes and lipid profiles. Oxidative stress parameters were investigated by their concentrations in the plasma and liver tissue of all study groups.
RESULTS
Spectroscopic analysis revealed that the total phenolic and flavonoid contents of DM extract were 69.48 mg Gallic Acid Equivalents per gram (GAE/g) and 49.59 mg Catechin Equivalents per gram (CE/g), respectively. Gallic acid (6.89 mg/g) and rutin (24.78 mg/g) were identified as the predominant phenolic compounds. The extract exhibited 2,2-Diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity with an IC50 of 179 µg/mL compared to 20.65 µg/mL for BHT.
DISCUSSION
Treatment with DM significantly (p < 0.05) decreased serum cholesterol, total protein, and triglyceride levels in LDDM-INH+RIF and HDDM-INH+RIF groups relative to hepatotoxic control. Similarly, plasma liver function enzymes were reduced in a dose-dependent manner. Antioxidant analysis indicated a significant (p < 0.05) elevation of superoxide dismutase (SOD) and glutathione (GSH), accompanied by a significant decline in malondialdehyde (MDA) levels in both plasma and liver tissues compared with the disease control group.
CONCLUSION
DM exhibited significant hepatoprotective effects in isoniazid- and rifampicininduced hepatotoxic rats, primarily through its antioxidant activity. The extract enhanced hepatic and plasma antioxidant enzymes (SOD, GSH) while reducing lipid peroxidation (MDA). It also normalized liver function markers, cholesterol, and triglyceride levels, consistent with histopathological improvements. These protective effects are attributed to the phenolic and flavonoid constituents of the extract.
Bushra Shaukat, Md. Mujahid, M. Rahman et al.· Recent advances in inflammat...· 0 citations
The estimated biomarkers provide evidence that the Lannea coromandelica bark extract has shown hepatoprotective activity against paracetamol induced hepatotoxicity in rats.
Md Rashed Hossen, Khaleda Nahid, M. Rahman et al.· Bangladesh Pharmaceutical Jo...· 0 citations
Liver diseases are a major global health burden, and oxidative stress plays
a key role in the development of hepatocellular injury. Mimusops elengi L. fruits contain bioactive
phytochemicals with reported antioxidant properties. This study aimed to evaluate the hepatoprotective
activity of aqueous Mimusops elengi fruit extract against carbon tetrachloride (CCl4)-induced
liver injury in Wistar rats.
Male Wistar rats were randomly allocated into five groups (n = 5). Hepatic injury was
induced using CCl4 (1 mL/kg, i.p.) for 15 days. Animals received Mimusops elengi fruit extract (200
and 400 mg/kg, orally) or silymarin (25 mg/kg). Serum ALT, AST, and catalase activity were evaluated,
followed by histopathological examination of liver tissues.
CCl4 administration significantly increased serum ALT and AST levels while reducing catalase
activity, indicating severe hepatic injury. Treatment with Mimusops elengi fruit extract significantly
reduced liver enzyme levels and improved antioxidant status in a dose-dependent manner.
Histopathological examination demonstrated preservation of hepatic architecture, reduced necrosis
and decreased inflammatory changes, particularly at 400 mg/kg.
The hepatopr tective activity observed may be attributed to the antioxidant and membrane-
stabilizing properties of flavonoids, tannins, and saponins present in Mimusops elengi fruits.
The biochemical and histopathological findings collectively support the traditional medicinal use of
this plant for liver protection and suggest its potential as a natural hepatoprotective agent.
Mimusops elengi fruit extract exhibits significant hepatoprotective effects against
CCl4-induced liver injury, likely mediated through antioxidant mechanisms. These findings support
its potential as a natural therapeutic agent, although further studies are required to elucidate its active
constituents and molecular pathways.
Nisha Chandila, Suresh Kumar, Manoj Kashyap· The International Journal of...· 0 citations
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