Aug 2026· Journal of Clinical and Diagnostic Research· 0 citations
TL;DR
The AETP flower extract demonstrates dosedependent protective effects on the liver against PCM-induced liver injury, likely attributed to its antioxidant and anti-inflammatory phytochemicals.
Abstract
Introduction: Paracetamol (PCM) overdose is a major contributor to acute liver failure, mainly resulting from oxidative stress and the depletion of Glutathione (GSH) triggered by its toxic byproduct, N-Acetyl-P-Benzoquinone Imine (NAPQI). Conventional hepatoprotective agents have limitations, prompting interest in medicinal plants. Tagetes patula (French marigold), traditionally used for hepatic disorders, contains flavonoids and terpenoids with potential antioxidant activity, but its hepatoprotective effects remain underexplored.
Aim: To evaluate the hepatoprotective effect of Aqueous Extract of Tagetes patula flowers (AETP) against PCM-induced hepatotoxicity in rats.
Materials and Methods: This an experimental animal study was conducted at the Departments of Pharmacology and Pathology, Regional Institute of Medical Sciences (RIMS), Imphal, India from November 2021 to May 2022. A total of 25 adult male Wistar albino rats were divided into five groups (n=5). Group I received vehicle (control), Group II received PCM (2 g/kg; toxic control), Group III received PCM + silymarin (25 mg/kg; standard), and Groups IV and V received PCM + AETP (200 mg/kg and 400 mg/kg, respectively) for seven days. Serum AST, ALT, and ALP levels were measured, and liver histology was examined. Data were analysed using one-way Analysis of variance (ANOVA) with Bonferroni post-hoc test.
Results: The PCM caused significant elevation of AST, ALT, and ALP (p<0.001) and severe histopathological damage. Silymarin markedly reduced enzyme levels and preserved hepatic architecture. AETP treatment produced a dosedependent hepatoprotective effect: 200 mg/kg partially improved biochemical and histological parameters, while 400 mg/kg significantly reduced enzyme levels (p<0.05 vs toxic) and showed improved hepatic architecture.
Conclusion: The AETP flower extract demonstrates dosedependent protective effects on the liver against PCM-induced liver injury, likely attributed to its antioxidant and anti-inflammatory phytochemicals. Additional research is required to elucidate its underlying mechanisms and validate its therapeutic efficacy
The estimated biomarkers provide evidence that the Lannea coromandelica bark extract has shown hepatoprotective activity against paracetamol induced hepatotoxicity in rats.
Md Rashed Hossen, Khaleda Nahid, M. Rahman et al.· Bangladesh Pharmaceutical Jo...· 0 citations
It is indicated that combining papaya, neem, and garlic extracts may provide greater hepatoprotection than administering the individual extracts against DEN-induced liver injury, and supports further mechanistic and preclinical evaluation of this polyherbal formulation before its potential therapeutic application in liver-disease management.
Egeonu Us, Umaru I. J., Izam Ed et al.· African Journal of Clinical...· 0 citations
Background: Drug-induced liver injury (DILI) is a major cause of hepatic morbidity. Isoniazid (INH), a first-line
antitubercular drug, is frequently associated with hepatotoxicity due to the formation of reactive metabolites that
deplete hepatic antioxidant defenses. Plant-derived antioxidants may offer protection against such injury.
Objective: To evaluate the hepatoprotective potential of the ethanolic seed extract of Coix lacryma-jobi L. (CLJSE)
against isoniazid-induced hepatotoxicity using in vitro and in vivo models.
Methods: CLJSE was prepared by Soxhlet extraction using 70% ethanol and standardized through pharmacognostic,
physicochemical, phytochemical, and FTIR analyses. Antioxidant activity was assessed by the DPPH assay, while
hepatoprotective activity was evaluated in HepG2 cells against H₂O₂-induced cytotoxicity using the MTT assay. Acute
oral toxicity was conducted according to OECD Guideline 423. For the in vivo study, Wistar rats were divided into
normal control, toxic control (INH, 50 mg/kg i.p., twice weekly for 28 days), standard (INH + silymarin 100 mg/kg
p.o.), and extract-treated groups (INH + CLJSE 200 or 400 mg/kg p.o.). Serum liver markers, hepatic antioxidant
parameters, liver weight, and histopathology were evaluated.
Results: CLJSE showed concentration-dependent DPPH radical scavenging activity (IC₅₀ = 74.56 µg/mL) and
protected HepG2 cells against oxidative stress, producing 94.46% cell viability at 100 µg/mL. The extract was nontoxic up to 2000 mg/kg. INH significantly increased serum AST, ALT, ALP, total bilirubin, liver weight, and MDA
levels, while reducing GSH, SOD, and CAT levels (p < 0.01). CLJSE treatment significantly and dose-dependently
reversed these changes, with the 400 mg/kg dose showing effects comparable to silymarin. Histopathological findings
confirmed marked protection of hepatic architecture.
Conclusion: CLJSE exhibits significant dose-dependent hepatoprotective activity against isoniazid-induced liver
injury, primarily through antioxidant, anti-lipid-peroxidative, and membrane-stabilizing mechanisms, supporting its
potential as an adjuvant phytotherapeutic agent during antitubercular therapy.
Soniya C. Deshmukh, K. Aghade, Nitin P. Jain et al.· International Journal of Dru...· 0 citations
Findings suggest that the hepatoprotective effect of HAEIB may be attributed to its antioxidant phytoconstituents and support its potential use as a natural hepatoprotective agent.
Shweta Patil, A. Sachin, Pratiksha Vadhane et al.· Genetics and Molecular Resea...· 0 citations
Under the experimental conditions, the individual ethanolic extracts, particularly O. gratissimum, were associated with improved hepatorenal biochemical indices, whereas combined administration was associated with marked hepatic.
C. G. Ehie, Ahamefula Sunday Ezekwe, A. Okari et al.· Health Science Research Inte...· 0 citations
INTRODUCTION/OBJECTIVE
Digera muricata (DM) contains phenolic compounds and quercetin and β-caryophyllene, which exhibit anticancer, anti-inflammatory, and antioxidant activities. The present study was conducted with the objective of examining the hepatoprotective role of DM ethanolic extract in a hepatotoxic model of Sprague-Dawley rats.
METHODS
Sprague-Dawley (SD) male rats were divided into the following groups: Normal Control (NC); hepatotoxic disease control (DC); hepatotoxic group treated with a low dose (LD) of DM (LDDM-INH+RIF); hepatotoxic group treated with a high dose (HD) of DM (HDDMINH+RIF), and reference control (RC) standard drug silymarin for 21 days. The hepatoprotective action was explored by measuring various liver function enzymes and lipid profiles. Oxidative stress parameters were investigated by their concentrations in the plasma and liver tissue of all study groups.
RESULTS
Spectroscopic analysis revealed that the total phenolic and flavonoid contents of DM extract were 69.48 mg Gallic Acid Equivalents per gram (GAE/g) and 49.59 mg Catechin Equivalents per gram (CE/g), respectively. Gallic acid (6.89 mg/g) and rutin (24.78 mg/g) were identified as the predominant phenolic compounds. The extract exhibited 2,2-Diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity with an IC50 of 179 µg/mL compared to 20.65 µg/mL for BHT.
DISCUSSION
Treatment with DM significantly (p < 0.05) decreased serum cholesterol, total protein, and triglyceride levels in LDDM-INH+RIF and HDDM-INH+RIF groups relative to hepatotoxic control. Similarly, plasma liver function enzymes were reduced in a dose-dependent manner. Antioxidant analysis indicated a significant (p < 0.05) elevation of superoxide dismutase (SOD) and glutathione (GSH), accompanied by a significant decline in malondialdehyde (MDA) levels in both plasma and liver tissues compared with the disease control group.
CONCLUSION
DM exhibited significant hepatoprotective effects in isoniazid- and rifampicininduced hepatotoxic rats, primarily through its antioxidant activity. The extract enhanced hepatic and plasma antioxidant enzymes (SOD, GSH) while reducing lipid peroxidation (MDA). It also normalized liver function markers, cholesterol, and triglyceride levels, consistent with histopathological improvements. These protective effects are attributed to the phenolic and flavonoid constituents of the extract.
Bushra Shaukat, Md. Mujahid, M. Rahman et al.· Recent advances in inflammat...· 0 citations
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