Biomolecular Condensates Dictate the Folding Landscape of Protein Alpha-Helices.

Abstract

Protein structure is exquisitely sensitive to the surrounding chemical environment, and many proteins encounter complex environments within cells. Importantly, numerous proteins organize into biomolecular condensates─dense macromolecular assemblies with distinct physicochemical properties. This raises a fundamental question: how do condensates reshape protein structure and dynamics? Here, we investigate how protein folding landscapes are altered inside condensates, using the protein α-helix as a model folded domain. Atomistic simulations suggest the helix-coil transition within condensates differs markedly from its behavior in dilute solution or in the presence of inert crowders. We then use Bayesian optimization to develop a chemically specific, residue-resolution model for quantification of α-helical folding and apply it to characterize diverse helices, including α-helical domains from the disease-associated proteins TDP-43, Annexin A11, and Androgen Receptor, within condensates of varying physicochemical properties. Our results support a framework in which multivalent interactions drive unfolding while crowding promotes folding, and α-helix conformational ensembles inside condensates emerge from this balance. Additionally, we show that helix folding transitions are kinetically frustrated inside condensates because they are coupled to the time scale of contact rearrangement with co-condensate proteins. As such, α-helix folding landscapes within condensates are dually sequence-dependent, informed by both the sequence of the α-helical domain and co-condensate proteins. Together, our work has implications for understanding condensate-mediated proteinopathies, targeting aberrant condensates, and designing condensates to program protein function across scales.