[Mild solubilization, purification, and immunoreactivity characterization of VP1 inclusion body protein of serotype O foot-and-mouth disease virus based on sodium lauroyl sarcosinate].
Aug 2026· Sheng wu gong cheng xue bao = Chinese journal of biotechnology· Vol 42 8, pp.
3541-3549
· 0 citations
Medicine
TL;DR
The LSN-based mild solubilization and chromatographic purification process developed in this study successfully circumvents the destructive effects and complex refolding steps associated with conventional strong denaturants, and the prepared recombinant His-VP1 protein possesses the potential to serve as a high-quality serological diagnostic antigen.
Evidence is provided that ArcticExpress (DE3) enable the soluble production of full-length HuNoVs VP1 suitable for downstream immunochemical analysis and this study provides evidence that ArcticExpress (DE3) enable the soluble production of full-length HuNoVs VP1 suitable for downstream immunochemical analysis.
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