A) Western blot shows FGFR1 protein levels in WT-MCF7 and TR-MCF7 cells. GAPDH was as loading control. B) Relative expression of FGFR1 in WT-MCF7 and TR-MCF7 cells, measured by RT–qPCR and normalized to ACTB and RPLP1. Fold change was calculated relative to the geometric mean of ΔCq of the MCF7-WT condition. C) Western blot shows STING protein levels in WT-MCF7 and TR-MCF7. Total protein as loading control. D) Relative expression of STING1 in WT-MCF7 and TR-MCF7 cells measured by RT–qPCR and normalized to ACTB and RPLP1. Fold change was calculated relative to the geometric mean of ΔCq of the MCF7-WT condition. E–H) Relative expression of IFNA2 (E-F) and STING1 (G–H) in TR-T47DshCTRL/TR-T47DshFGFR1 cells and TR-MCF7shCTRL/TR-MCF7shFGFR1 cells respectively, treated with either H2O or poly(dA:dT), measured by RT–qPCR and normalized to GAPDH and RPLP1. Fold change was calculated relative to the geometric mean of ΔCq of the shCTRL/H2O condition. I) Relative expression of FGFR1 in WT-MCF7 and R1-MCF7 cells, measured by RT-qPCR and normalized to HPRT1.
The comparison of adopter and non-adopter sample reveals three potential adoption inhibitor, security, data privacy, and portability, which underlines the importance of the technical and security perspectives for research investigating the adoption of technology.
Nattakarn Phaphoom, Xiaofeng Wang, S. Samuel et al.· Journal of Systems and Softw...· 111 citations· ⚡8
This study investigates how Lean internal startup facilitates software product innovation in large companies and identifies its enablers and inhibitors, and shows the potential of the method-in-action framework to investigate the Lean startup approach in non-startup context.
Henry Edison, Nina M. Smørsgård, Xiaofeng Wang et al.· Journal of Systems and Softw...· 78 citations· ⚡6
This paper highlights the challenges to conduct proper affect-related studies with psychology, provides a comprehensive literature review in affect theory, and proposes guidelines for conducting psychoempirical software engineering.
D. Graziotin, Xiaofeng Wang, P. Abrahamsson· SSE@SIGSOFT FSE· 56 citations· ⚡4
This study conducts a multiple case study on twenty European software startups and proposes a prototype-centric learning model in early stage software startups, and identifies factors that occur as barriers but also facilitators for prototyping in earlystage software startups.
Anh Nguyen-Duc, Xiaofeng Wang, P. Abrahamsson· International Conference on...· 44 citations· ⚡5
It is demonstrated that linker-free PROTACs can outperform traditional designs, marking a paradigm shift in PROTAC development for targeted protein degradation.
Pinal, a 16-billion-parameter foundation model that produces protein candidates from natural-language functional descriptions, supports natural language as a high-level interface for candidate generation in protein design, enabling programmable exploration with reduced reliance on manually specified structural or seque...
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
