Large-scale structural analysis of pre-mRNA structure using RADIS

Sequence-intrinsic barriers define a class of translation-restricted uORFs in the human genome

This work systematically quantified the intracellular accumulation of 111 evolutionarily conserved human uORFps and identified a conserved class of human uORFs that encode intrinsic barriers to productive translation and provide a rigorous framework for understanding how noncanonical coding sequences shape the human pr...

FAST-MaP: Chemical Mapping of RNA Structures Using Primer-less Sequencing

RNA structure governs the function of non-coding RNAs and influences mRNA stability and translation, making testing of models of experimental RNA structure relevant to fields spanning structural biology, virology, and molecular therapeutics. Here we present FAST-MaP (Fast and Accessible Sequencing Technology for Mutati...

SPLISOFORMS: a Structure-Resolved Knowledge Base of Alternative Splicing Isoforms

Background Alternative splicing expands the coding capacity of single genes into diverse protein families, and its dysregulation is a recognized hallmark of cancer. Despite this, the characterization of splice variants is largely restricted to sequence-level annotations. The functional consequences of an isoform, such...

High-resolution mapping of RNA structural maturation during Cas9 assembly with ABEL-FRET

This work explored how the guide RNA of CRISPR genome editing system folds and modulates its structural flexibility to carry out the roles required for each assembly state from its unbound apo form to the functional Cas9 RNP state for target DNA cleavage using ABEL-FRET spectroscopy.

RNA engineering approaches to aid high-resolution structure determination of medium- to large-sized RNAs.

High-resolution structure determination of RNA remains a major challenge due to its conformational flexibility, intrinsic heterogeneity, and susceptibility to degradation. Although RNA plays central roles in gene regulation, viral replication, and cellular homeostasis, RNA-only structures represent a small fraction of...

The RNA structural code: orchestrating gene expression and enabling precision therapies.