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Efficient CRISPR/Cas9-mediated homology independent sequence replacement in vivo and non-dividing primary cells

Tu Dang Alexandra Roman Anja Zimmer M. Lebedin Ella Bahry Claudia Marti Grifol Markus Esser Seren Sevim Wunderlich Duncan Miller Sebastian Diecke Ralf Kühn
Jul 2026 · bioRxiv · 0 citations
Biology

TL;DR

REPLACE is established as a versatile platform for homology-independent sequence replacement and as a complementary approach for genetic correction in settings where homology-directed repair (HDR), base editing (BE) or prime editing (PE) are inefficient or not applicable.

DOI Semantic Scholar
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