Genome-Wide CRISPR Screening Identifies Cellular Factors Controlling Nonviral Genome Editing Efficiency

Shivani Saxena Meha Kabra Amr A. Abdeen Divya Sinha Min Zhu Ruo-Sen Xie Giovanni M. Hanstad Maria A. Fernandez Zepeda David M. Gamm B. Pattnaik Shao-Qin Gong Krishanu Saha

Mar 2025 · bioRxiv · 2 citations · 71 references

Biology Medicine

TL;DR

A novel genome-wide CRISPR screening strategy that will facilitate the systematic engineering of novel nonviral genome editing delivery methods, where the identified novel gene hits can be further used to increase editing efficiency for other therapeutically relevant cell types.

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