The archive contains the processed ¹⁵N CEST profiles of WT Im7 recorded at 600, 800 and 1200 MHz at 10 °C and at 800 MHz at 15 and 20 °C, the ¹⁵N TROSY-CPMG profiles recorded at 600 and 800 MHz at 10 °C, and the ChemEx experiment, method and parameter files together with the shell scripts that launched every fit report...
Ved Prakash Tiwari, Laetitia Rožić, Sebastian Hiller· Zenodo (CERN European Organi...· 0 citations
GHKL ATPases share a unique Bergerat ATP-binding fold and regulate diverse biological processes through ATP-dependent conformational changes. An early step of ATP hydrolysis in this family has been attributed to a single highly conserved glutamate residue proposed to function as the general base. However, mutations of...
Kenji Fukui, Ayaka Shibuya, Takeshi Murakawa et al.· 0 citations
GHKL ATPases share a unique Bergerat ATP-binding fold and regulate diverse biological processes through ATP-dependent conformational changes. An early step of ATP hydrolysis in this family has been attributed to a single highly conserved glutamate residue proposed to function as the general base. However, mutations of...
Kenji Fukui, Ayaka Shibuya, Takeshi Murakawa et al.· 0 citations
In this study, constructed rapid infiltration systems (CRIS) packed with coke or modified red mud were used to treat mineral processing wastewater (MPW) containing aniline aerofloat (AAF) and ammonium dibutyl dithiophosphate (ADD) at 10 °C. Microbial community dynamics and metagenomics were analyzed to investigate eluc...
Shenglong Chen, Shuying Wang, Qiuhong Li et al.· Journal of Water Process Eng...· 0 citations
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It is indicated that cellular adaptation is insufficient to restore mineralization in the absence of PHOSPHO1, and instead, PHOSPHO1 appears to play a critical role in establishing the molecular and biophysical properties of MVs necessary for mineral nucleation and propagation.
L. H. S. Andrilli, L. Hayann, C. Tokuhara et al.· Bone· 0 citations
Hexavalent chromium [Cr(VI)] and acenaphthene (Ace) can co-occur in industrially affected aquatic environments, but their effects across target organs under co-exposure remain poorly characterized. Adult male zebrafish were exposed for 28 d to environmentally relevant or high sublethal concentrations of Cr(VI) and Ace,...
The tremendous therapeutic potential of carbon-based nanomaterials (CNMs) has been limited by inconsistent data regarding the nanotoxicity assessment. Although a bulk of studies have been performed to assess the in vitro cytotoxicity mechanism of CNMs, the exact factors responsible for the cytotoxicity of CNMs have not...
Ayimgul Uzunyol, Nedim Hacıosmanoğlu, Fatih Inci· Journal of Applied Toxicolog...· 0 citations
Cyclic nucleotide second messengers are used in all domains of life to amplify viral infection signals and activate cellular defences. In prokaryotes, CBASS (cyclic nucleotide-based antiphage signalling system) and type III CRISPR-Cas systems generate a range of cyclic nucleotides which bind and allosterically activate...
S. Grüschow, Peter Wotherspoon, Emma Hilton-Balfe et al.· PLoS Biology· 1 citation· ⚡1
Proteome-scale screening is attractive for polypharmacology and safety assessment but is often dominated by computationally expensive docking and opaque target-prediction models. We developed a ligand-conditioned pocket-transfer workflow that combines public chemical structures, experimentally observed template ligands...
Ying Ye· Zenodo (CERN European Organi...· 0 citations
The inclusion of non-proteinogenic amino acids (npAAs) into proteins vastly expands their chemical repertoire and hence possible functional diversity. However, it remains challenging to obtain non-proteinogenic sequences that carry out a specific task. Here we develop a platform to discover functional non-standard prot...
Adam T. Beattie, Alexander A. Vinogradov, Chikako Okada et al.· Angewandte Chemie· 0 citations
Background: The advent of cystic fibrosis transmembrane conductance regulator protein (CFTR) modulators like ivacaftor have revolutionised the treatment of cystic fibrosis (CF). However, due to the plethora of variances in disease manifestations in CF, there are inherent challenges in unified responses under CFTR modul...
Tony Velkov, Gauri G. Rao, Helmut Ellemunter et al.· UNC Libraries· 0 citations
Efficient catalysis often requires high local concentrations of reactants and catalysts, which cells achieve through compartmentalization within organelles, such as carboxysomes, that increase the efficiency of bacterial carbon fixation. Here, we engineered a photocatalytic reaction compartment that concentrated an art...
Abesh Banerjee, R. C. Fabry, Giovanna Ghirlanda· bioRxiv· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
