Protein-based pharmaceuticals have become indispensable in modern medicine because of their high specificity and therapeutic efficacy. However, unlike conventional small-molecule drugs, proteins are inherently unstable and susceptible to both physical and chemical degradation during manufacturing, storage, and administ...
Nargeees Fathima Rameesa, Haris Hadiya, Rishal Mohammed et al.· Zenodo (CERN European Organi...· 0 citations
De novo protein design has advanced rapidly, yet designing binders to polar, solvent-exposed epitopes and small, flexible ligands remains challenging. Such hydrated surfaces and flexible molecules, including carbohydrates, provide few of the hydrophobic contacts favoured by current methods and have largely resisted de...
Kieran Didi, Danny Reidenbach, Matthew Penner et al.· bioRxiv (Cold Spring Harbor...· 0 citations
Due to genome segmentation, rotaviruses must co-package eleven distinct genomic RNAs. The packaging is mediated by virus-encoded RNA chaperones, such as the rotavirus NSP2 protein. While the activities of distinct RNA chaperones are well studied on smaller RNAs, little is known about their global effect on the entire v...
Daniel Desirò, Michael L. Knight, Anastacia Wienecke et al.· UNC Libraries· 0 citations
Protein-based pharmaceuticals have become indispensable in modern medicine because of their high specificity and therapeutic efficacy. However, unlike conventional small-molecule drugs, proteins are inherently unstable and susceptible to both physical and chemical degradation during manufacturing, storage, and administ...
Nargeees Fathima Rameesa, Haris Hadiya, Rishal Mohammed et al.· Zenodo (CERN European Organi...· 0 citations
Proteins are often composed of repeating sequences and structural units that govern folding, stability, molecular recognition, and catalysis. This review offers a comprehensive overview of such units, generally called motifs, and highlights recent advances in understanding their structural and functional roles. We disc...
Biomolecular condensates provide dynamic environments that store and organize proteins, yet the fundamental principles determining whether condensation promotes or suppresses irreversible protein aggregation remain unclear. Here, we show that the receptor-interacting protein kinase 3 (RIPK3) RHIM domain follows distinc...
Tian-Chen Li, Brayden C. Williams, Qi Han et al.· bioRxiv· 0 citations
Proteins are often composed of repeating sequences and structural units that govern folding, stability, molecular recognition, and catalysis. This review offers a comprehensive overview of such units, generally called motifs, and highlights recent advances in understanding their structural and functional roles. We disc...
Biallelic variants in retinol dehydrogenase 12 ( RDH12 ) cause severe inherited retinal diseases (IRDs) with no available treatments. Given the potential risks of subretinal delivery in patients with macular atrophy, we evaluated intravitreal AAV-mediated RDH12 gene augmentation. A Rdh12 -knockout rat model and patient...
Yunyu Zhou, Yamei Li, Yanan Shi et al.· Stem Cell Research & Therapy· 0 citations
Cell surface proteins represent an important source of biomarkers and therapeutic targets. However, due to the inherent sensitivity limitations of existing technologies, tissue and cell-type-specific surfaceomes remain poorly characterized, especially in the context of human diseases. Here, we present nanoMAPS (nanosca...
Amanda Lorentzian, Juliet M. Bartleson, Terence Ho et al.· Nature Communications· 0 citations
It is shown that splice-switching ASOs can elicit target exon skipping and up-regulate endogenous protein levels, and it is shown that TSC1 uORFs act additively to reduce protein translation.
Eloise S. Beer Wells, L. De Conti, Hyung Chul Kim et al.· Genome Medicine· 0 citations
A new machine-learning framework aims to improve the success rate of computational protein design while moving away from results that reproduce sequences found in nature.
