protein folding

Brown and white adipocyte like cells derived exosomes differentially regulate stemness, hormone secretion, apoptosis, and lipid metabolism in model cells

Assessment of the impact of conditioned media or exosomes derived from brown and white adipocyte-like cells on four human cell types highlighted a potential modulatory role of adipocyte-derived exosomes in intercellular communication.

Leveraging Protein Dynamics for Selective Inhibition of Threonyl‐tRNA Synthetase by Obafluorin Analogs

The widespread emergence of antibiotic resistance necessitates the development of novel agents with unique mechanisms of action. Obafluorin (OB), a natural β-lactone antibiotic, is a covalent inhibitor of threonyl-tRNA synthetase (ThrRS), but the high conservation of the active site between prokaryote and eukaryote Thr...

Evolutionary origins of protein novelty across an entire yeast subphylum

A broad computational investigation of genetic and protein novelty throughout the entire subphylum of Saccharomycotina yeasts shows that “invention” of new folds occurs more frequently through de novo emergence, and illustrates cases of evolutionary “re-invention” of existing protein folds from noncoding sequences.

Multicomplex Integrative Structural Modeling of a Human Histone Deacetylase Interactome

Histone Deacetylase (HDAC) 1 and 2 are key enzymatic components in multiple large chromatin remodeling complexes including NuRD, SIN3, and CoREST. In addition, both HDAC 1 and 2 contain a large intrinsically disordered region (IDR) within their C-terminal domain (CTD). How HDAC1/2 assemble into these complexes and the...

Funneliformismosseae symbiosis and TiO2 nanoparticles under drought in Melissa officinalis L.: a machine learning modeling approach

Plant-mycorrhizal symbiosis under combined abiotic stress and nanoparticle exposure remains underexplored. The complexity of such multifactorial systems, combined with the labor-intensive nature of physiology study, challenges conventional analytical approaches. Machine learning can contribute to further elucidating re...

Sodium butyrate improves growth, antioxidant capacity and intestinal health in juvenile common carp fed high-plant-protein diets

ENGINEERING 3'-UTR HAIRPIN STRUCTURES TO MODULATE MRNA STABILITY AND RECOMBINANT PROTEIN PRODUCTION IN ESCHERICHIA COLI.

Engineering mRNA stability is a promising yet underexplored approach for improving recombinant protein production in bacterial systems. In this study, we evaluated the effect of synthetic 3'-UTR hairpin structures on mRNA stability and protein yield in Escherichia coli using two SUMO-fusion expression systems. Hairpin...

Principles of Co-Transcriptional Ribosome Assembly Nucleation and Quality Control

The ribosome is a universally conserved macromolecular machine responsible for protein synthesis across all domains of life. It is composed of two subunits, the small ribosomal subunit (SSU) and the large ribosomal subunit (LSU), which come together during translation to form the functional ribosome. In Saccharomyces c...

Identification of genetic determinants that promote biofilm growth under heterotrophic conditions in Cupriavidus necator using transposon enrichment

Results identify B2043 as a negative regulator of biofilm formation acting predominantly during attachment, provide the first experimental evidence for c-di-GMP-dependent biofilm regulation in C. necator H16, and establish a functional-genomics framework — together with eleven further candidate loci — for engineering p...

Fractal Compact Manifold Theory Core Mechanics: Multifractal Unification of Quantum Mechanics, Gravity, and Consciousness

Abstract This sub-paper, "Fractal Compact Manifold Theory Core Mechanics" serves as a concise, standalone distillation of FCMT's foundational elements, emphasizing the atemporal symmetry breaking, field emergence, Lagrangian formulation, projection operator, and foliation map. By focusing solely on these core mechanics...

Micro- and macro- heterogeneity of N-glycoproteome remodeling in endoplasmic reticulum stress

N-glycosylation plays essential roles in the folding, trafficking, and maturation of proteins in the secretory pathways, but how individual protein- and site- specific glycosylation rewires under endoplasmic reticulum (ER) stress is unknown. Particularly, intact glycopeptide data that retain the connectivity between gl...

Effectiveness, Patient Satisfaction, and Safety of a Next-generation PLLA Collagen Biostimulator for Nasolabial Fold Augmentation: 12-month Results of a Prospective Multicenter Study.

JULÄINE produced sustained, clinically meaningful improvements in patient-perceived aesthetic outcomes with high treatment satisfaction over 12 months, and the consistent perception of natural-looking results is in line with the progressive, collagen-building mechanism of PLLA-LASYNPRO.