protein folding

REM Gene Family in Sugar Beet and Functional BvREM12 in Salt Stress Tolerance

Soil salinization acts as a major abiotic stress and severely hampers global agricultural productivity. Elucidating the molecular mechanisms underlying plant salt tolerance is of great significance for crop improvement. Remorin (REM) proteins are plant-specific membrane marker proteins that play a key role in stress si...

Joint optimisation of amino acid and coding sequence for de novo designed proteins

Deposit formation in TGFBI corneal dystrophy as interface-limited precipitation on a non-renewing layer: model code and digitised source data

Model code, digitised source data and figure scripts for a forward model of deposit formation in granular corneal dystrophy type 2 (TGFBI R124H), treated as precipitation of a slightly supersaturated protein in a curved, partly non-renewing tissue rather than as a protein-folding disease. The work has no experimental d...

Smartphone-Assisted Electrochemiluminescence Imaging Platform Based on Protamine-Enhanced Gold Nanoclusters for Ultrasensitive and Visualized Detection of Zearalenone

Zearalenone (ZEN) contamination in grains poses a serious threat to human and animal health, yet existing imaging-based platforms for rapid visualized detection remain limited by insufficient sensitivity, limited luminescence efficiency, and poor signal stability, hindering further practical applications. Herein, a p...

Enhancing protein structure prediction accuracy by prioritizing important residues using protein language models

Accurate prediction of protein tertiary structures from amino acid sequences remains a fundamental challenge in computational biology. Although AlphaFold2 represents a major advance, systematic discrepancies persist between its predictions and experimentally determined structures. Given that individual residues contrib...

Explainable ML force-field for evaluating protein–ligand binding energy using SO3LR

Accurate estimation of protein–ligand binding affinities remains central to structure-based drug design. Classical force fields neglect important quantum-mechanical effects, while quantum mechanical and semi-empirical methods remain impractical for large biomolecular systems. Machine-learned force fields (MLFFs) offer...

Ultrasound-Synchronized In Situ Crystallization Locks Transient High-Activity Enzyme Conformations within Covalent Organic Frameworks

Enzymes transiently access high-activity conformations with exposed cryptic sites and heightened substrate affinity, yet these states dissipate upon relaxation to the thermodynamic ground state once the activating perturbation is removed, leaving this catalytic potential largely inaccessible. Here we show that such f...

Comparative proteomic and metabolomic profiling of transparent versus opaque pigeon egg albumen.

BACKGROUND Pigeon albumen exhibits superior gel properties and a characteristic translucent appearance, yet substantial variation in albumen transparency exists among individual eggs. The molecular basis underlying this quality difference remains poorly understood, particularly regarding protein and metabolite composit...

Sero-prevalence, risk factor and force of infection estimates for foot-and-mouth disease in two cattle rearing areas of Cameroon

Foot-and-mouth disease continues to be a major disease burden globally, directly impacting endemic regions and indirectly affecting free regions. There is a lack of reliable estimates of key parameters delivered from empirical field data, such as transmission rates for developing useful models to assess the impacts of...

Alternative genetic codes in bacteria and archaea identified with a fast k-mer-based algorithm.

The genetic code is conserved across all domains of life and is often described as universal. Nevertheless, many exceptions to the "universal" code have now been documented, most of these through manual or semiautomated inspection of highly conserved genes. Modern bioinformatics tools improved our ability to find alter...

Longitudinal repeated protein measurements in a multiethnic cohort identify novel diabetes biomarkers that reveal unique disease pathways

Circulating diabetes biomarkers have been identified with proteomics measured at a single timepoint, but what is additionally provided by longitudinal repeated measurements in the same individuals, over many years, is unknown. We studied participants in the Multi-Ethnic Study of Atherosclerosis (MESA; N=5,322, mean bas...

Direct-to-biology approach for the selective identification of ligands for alpha-synuclein aggregates

The selective targeting of α-synuclein (α-syn) aggregates represents a critical challenge for the diagnosis and treatment of Parkinson’s disease and related synucleinopathies. While several ligands have been developed for amyloid imaging, most of them lack sufficient specificity for α-syn over other amyloid fibrils. We...