gene editing

A Zero-Shot Single-Point Chemical Language Model Mimics Medicinal Chemist Reasoning for Molecular Optimization and STING Inhibitor Development in Alzheimer’s Disease

Drug development relies heavily on the ability of medicinal chemists to identify productive, localized structural modifications that improve potency, selectivity, and developability while preserving favorable features of a lead scaffold. Here, we introduce the Single-Point Chemical Language Model (SpCLM), a zero-shot m...

Supplementary Datasets underlying analysis and results presented in CRISPR-enhanced assessment of variants of unknown significance nominates oncology therapeutic targets and drug repositioning opportunities. Savino et Al. 2026 and Data Package to reproduce results and figures.

Interpreting infrequent somatic variants remains a challenge in cancer genomics. We developed CRISPR-VUS, a framework using public Cancer Dependency Map data to identify Dependency-Associated Mutations (DAMs) - variants linked to increased host-gene dependency - with resolution extending to singleton events. Analysis o...

Editorial: Growth and development in horticultural crops: mechanisms, regulation, and innovation

Fruit ripening and quality. Fruit ripening is a tightly regulated developmental transition with direct implications for postharvest shelf life, nutritional value, and consumer acceptance. Three studies illuminate novel regulatory nodes in tomato and strawberry. Sun et al. (https://doi.org/10.3389/fpls.2025.1649082) dem...

Editorial: Genomic insights and molecular genetics of plant-nematode interactions

worldwide, causing substantial economic losses and posing a challenge to sustainable crop and forestry production [1]. The successful parasitism of PPNs is the outcome of a molecular dialogue with their hosts -as they exploit molecules and modulate host cellular processes for their own benefit. In response, plant hosts...

mRNA editing of the Alzheimer’s risk gene APOE

It is reported that APOE mRNA is enzymatically edited to convert E4 to E3/E2, and/or E2 to E3/E4, and up to ∼10% conversion was seen in brain, and up to 100% in vitro driven by the RNA-editing enzyme APOBEC1.

Charge modulation of peptide/nucleic acid complexes: an anionic additive enhances gene silencing and CRISPR/Cas9 editing by promoting intracellular nucleic acid release

Small interfering RNA (siRNA) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated Protein 9 (Cas9) complexes are effective approaches to temporarily downregulate protein expression via post-transcription RNA interference or permanently altering protein expression via editing gen...

Anthropological Singularity and Algorithmic Stratification: A Medical-Sociological Reframing of the Bioethical Principles Governing the Use of Artificial Intelligence in Healthcare

It is argued that algorithmic bias is part of socio-technical ecosystems, being a systemic property of these and that responsibility must migrate towards distributed frameworks between human and non-human agents, and that the digital revolution could produce an algorithmic stratification of populations, now prolonged t...

Navigating off-target effects in CRISPR-based genome editing for safer gene therapies

Off-target activity remains a significant challenge in the clinical development of CRISPR-based therapies. As programmable nucleases advance from experimental tools toward approved medicines, the ability to predict, detect, and mitigate unintended genomic editing events has acquired direct translational importance. Thi...

Peripheral Blood Mononuclear Cell Transcriptomics in Porcine Reproductive and Respiratory Syndrome: A Window into Innate Immune Resistance and Tolerance to Viral Disease

Peripheral blood mononuclear cells (PBMCs) are the most immunologically active and readily accessible fraction of whole blood, and they initiate host immune responses following infection and vaccination. Since PBMC transcriptomes capture diverse host–pathogen interactions, they offer a promising tool to uncover the gen...

In silico identification and structural characterization of ClAPRR2 and ClPSY1 as independent genetic targets for a white-rinded, orange-fleshed watermelon (Citrullus lanatus) accessible to colour-vision-deficient consumers

Supplementary Datasets and Code Package for CRISPR-enhanced assessment of variants of unknown significance nominates oncology therapeutic targets and drug repositioning opportunities Savino et Al. 2026

Interpreting infrequent somatic variants remains a challenge in cancer genomics. We developed CRISPR-VUS, a framework using public Cancer Dependency Map data to identify Dependency-Associated Mutations (DAMs) - variants linked to increased host-gene dependency - with resolution extending to singleton events. Analysis o...

Genetic Approach to Improving Bioremediation Strategies

Bioremediation, a sustainable and eco-friendly approach to environmental cleanup, has seen significant advancements through genetic engineering techniques. This chapter explores the pivotal role of genetic strategies in enhancing bioremediation efficacy. The introduction elucidates the fundamental principles of bioreme...