gene editing

Morphological and biochemical evaluation of tolerance to water deficit identifies SlCLE9 as a novel regulator of drought stress

We performed a screening to evaluate the responses to water deficit before the flowering stage in a panel of 31 genotypes composed of one commercial hybrid, one wild species, multiple ex-PVP (vintage) lines, several commercial heirlooms from the Northeast USA, Mexican landraces from hot and humid environments and genom...

Arrhythmogenic Cardiomyopathy

Arrhythmogenic cardiomyopathy (ACM) is an inherited myocardial disease characterized by progressive fibro-fatty replacement of the ventricular myocardium, predisposing to ventricular arrhthmias, heart failure, and sudden cardiac death. Historically described as arrhythmogenic right ventricular cardiomyopathy (ARVC), AC...

CanProTarget

A residue-resolved prioritization platform for covalent cancer drug discovery. CanProTarget integrates cancer gene dependencies, small-molecule covalent ligand engagement measured by chemoproteomics, and base-editing evidence for individual cysteines, keeping each layer at the resolution it is measured at.

EDITING OUT CARDIOVASCULAR RISK: A NARRATIVE REVIEW OF GENE-EDITING APPROACHES TO PCSK9

Background: PCSK9 is a circulating protease that regulates plasma low-density lipoprotein (LDL) cholesterol levels. It binds to the LDL receptor on the surface of hepatocytes and directs the receptor toward intracellular degradation, so that greater PCSK9 activity leaves fewer LDL receptors available to clear LDL-C fro...

Does adjusting for tumor proliferation bias prognostic gene expression estimates? Preregistered analysis code, decision rules and results

R analysis code, preregistered decision rules, result files, run logs and figures for a study of what omitting tumor proliferation does to prognostic gene expression estimates. For every expressed gene in a cohort, two Cox models are fitted for overall survival, one adjusted for age, sex and stage and one that adds a s...

Supporting data for “Comparative Genomics in the genus Caenorhabditis and the Discovery of a Novel Toxin-Antidote System in C. elegans”

This dataset accompanies the thesis “Comparative Genomics in the genus Caenorhabditis and the Discovery of a Novel Toxin-Antidote System in C. elegans.” It contains 37 files documenting the comparative genomic analyses and experimental work presented in the thesis. Main and supplementary figures cover genetic mapping,...

Stargardt disease: clinical studies over the past 5 years—a systematic review

Although STGD1 remains a challenging condition, new therapies in this review have shown potential in clinical trials, even though challenges such as off-target effects and DNA repair errors remain.

CRISPR/Cas9-mediated multicopy δ-site integration in Saccharomyces cerevisiae via Cas9 expression

Editorial: Education in synthetic biology

Synthetic Biology (SB) has emerged as a highly innovative field over the last two decades, bringing with it a vast array of tools, objectives, and significant recognition within the broader scientific community. As the field has grown, it has attracted researchers from a wide range of disciplines, including biology, bi...

RsHSFA3 of Rhododendron simsii confers plant heat tolerance by molecular regulation.

Physiological measurements demonstrated that RsHSFA3 positively regulates heat tolerance by boosting reactive oxygen species (ROS) scavenging capacity and preserving cell membrane stability, and synergistically enhances heat tolerance in R. simsii via activating the expression of heat shock proteins and facilitating RO...

Modeling Liver Cancer Molecular and Histological Features by CRISPR Editing of Porcine Hepatocytes.

Through ex vivo CRISPR editing of clinically relevant genes in porcine hepatocytes, ex vivo CRISPR editing of clinically relevant genes in porcine hepatocytes established cell and animal models that recapitulate key molecular and histological features of human HCC.

Adult-onset deficiency of β-klotho in the liver reveals a novel role in lipid homeostasis.

Using an adult-onset liver-specific knockout model, KLB function is disentangled from developmental confounders and established KLB as a critical regulator of adult lipid and lipoprotein homeostasis.